Construct: ORF TRCN0000470102
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002907.1_s317c1
- Derived from:
- ccsbBroadEn_10810
- DNA Barcode:
- ATAGCCTCATCACGTTTCCTCCGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ETV4 (2118)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470102
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2118 | ETV4 | ETS variant transcription f... | NM_001261439.3 | 100% | 100% | |
| 2 | human | 2118 | ETV4 | ETS variant transcription f... | XM_024450644.1 | 93.6% | 93.6% | 1_42del |
| 3 | human | 2118 | ETV4 | ETS variant transcription f... | NM_001261437.3 | 46.5% | 46.5% | 1_714del |
| 4 | human | 2118 | ETV4 | ETS variant transcription f... | NM_001261438.3 | 46.5% | 46.5% | 1_714del |
| 5 | human | 2118 | ETV4 | ETS variant transcription f... | NM_001369367.2 | 42.8% | 42.8% | 1_828del |
| 6 | human | 2118 | ETV4 | ETS variant transcription f... | NM_001079675.5 | 42.7% | 42.7% | 1_831del |
| 7 | human | 2118 | ETV4 | ETS variant transcription f... | NM_001369366.2 | 42.7% | 42.7% | 1_831del |
| 8 | human | 2118 | ETV4 | ETS variant transcription f... | NM_001986.4 | 42.7% | 42.7% | 1_831del |
| 9 | human | 2118 | ETV4 | ETS variant transcription f... | NM_001369368.2 | 41.7% | 41.7% | 1_831del;870_871insGGTGACGGGGCCATG |
| 10 | mouse | 18612 | Etv4 | ets variant 4 | NM_001316366.1 | 38.7% | 41.2% | (many diffs) |
| 11 | mouse | 18612 | Etv4 | ets variant 4 | NM_008815.3 | 38.3% | 40.8% | (many diffs) |
| 12 | mouse | 18612 | Etv4 | ets variant 4 | NM_001316365.1 | 38.2% | 40.7% | (many diffs) |
| 13 | mouse | 18612 | Etv4 | ets variant 4 | XM_006532462.3 | 38.1% | 40.6% | (many diffs) |
| 14 | mouse | 18612 | Etv4 | ets variant 4 | XM_006532461.2 | 35.3% | 37.6% | (many diffs) |
| 15 | mouse | 18612 | Etv4 | ets variant 4 | XM_006532460.2 | 35.2% | 37.5% | (many diffs) |
| 16 | mouse | 18612 | Etv4 | ets variant 4 | XM_006532459.2 | 34.9% | 37.2% | (many diffs) |
| 17 | mouse | 18612 | Etv4 | ets variant 4 | XM_006532458.2 | 34.8% | 37.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 687
- ORF length:
- 621
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta cctccacaca gagggcttct ctgggccctc tccaggtgac ggggccatgg 121 gctatggcta tgagaaacct ctgcgaccat tcccagatga tgtctgcgtt gtccctgaga 181 aatttgaagg agacatcaag caggaagggg tcggtgcatt tcgagagggg ccgccctacc 241 agcgccgggg tgccctgcag ctgtggcaat TTCTGGTGGC CTTGCTGGAT GACCCAACAA 301 ATGCCCATTT CATTGCCTGG ACGGGCCGGG GAATGGAGTT CAAGCTCATT GAGCCTGAGG 361 AGGTCGCCAG GCTCTGGGGC ATCCAGAAGA ACCGGCCAGC CATGAATTAC GACAAGCTGA 421 GCCGCTCGCT CCGATACTAT TATGAGAAAG GCATCATGCA GAAGGTGGCT GGTGAGCGTT 481 ACGTGTACAA GTTTGTGTGT GAGCCCGAGG CCCTCTTCTC TTTGGCCTTC CCGGACAATC 541 AGCGTCCAGC TCTCAAGGCT GAGTTTGACC GGCCTGTCAG TGAGGAGGAC ACAGTCCCTT 601 TGTCCCACTT GGATGAGAGC CCCGCCTACC TCCCAGAGCT GGCTGGCCCC GCCCAGCCAT 661 TTGGCCCCAA GGGTGGCTAC TCTTACTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 721 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 781 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAATAGCCTC 841 ATCACGTTTC CTCCGAACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 901 aagatt