Construct: ORF TRCN0000470107
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008245.1_s317c1
- Derived from:
- ccsbBroadEn_07793
- DNA Barcode:
- GAAATTCAATCTAGGGCCCATTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KLF12 (11278)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470107
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11278 | KLF12 | Kruppel like factor 12 | NM_007249.4 | 99.9% | 100% | 840C>A |
| 2 | human | 11278 | KLF12 | Kruppel like factor 12 | XM_005266251.4 | 99.9% | 100% | 840C>A |
| 3 | human | 11278 | KLF12 | Kruppel like factor 12 | XM_011534909.2 | 99.9% | 100% | 840C>A |
| 4 | human | 11278 | KLF12 | Kruppel like factor 12 | XM_011534910.2 | 97.6% | 97.2% | (many diffs) |
| 5 | human | 11278 | KLF12 | Kruppel like factor 12 | XM_017020384.1 | 97.4% | 97.2% | 0_1ins27;3_5delGGTinsAAA;813C>A |
| 6 | human | 11278 | KLF12 | Kruppel like factor 12 | XM_011534907.2 | 95.9% | 94% | (many diffs) |
| 7 | human | 11278 | KLF12 | Kruppel like factor 12 | XM_011534911.2 | 95.1% | 95.2% | 0_1ins57;783C>A |
| 8 | human | 11278 | KLF12 | Kruppel like factor 12 | XM_011534912.2 | 95.1% | 95.2% | 0_1ins57;783C>A |
| 9 | human | 11278 | KLF12 | Kruppel like factor 12 | XM_017020385.1 | 87.4% | 87.5% | 0_1ins150;690C>A |
| 10 | human | 11278 | KLF12 | Kruppel like factor 12 | XM_011534908.3 | 80.6% | 78.9% | (many diffs) |
| 11 | mouse | 16597 | Klf12 | Kruppel-like factor 12 | NM_010636.3 | 90.7% | 97% | (many diffs) |
| 12 | mouse | 16597 | Klf12 | Kruppel-like factor 12 | XM_006518623.3 | 90.7% | 97% | (many diffs) |
| 13 | mouse | 16597 | Klf12 | Kruppel-like factor 12 | XM_006518624.3 | 90.7% | 97% | (many diffs) |
| 14 | mouse | 16597 | Klf12 | Kruppel-like factor 12 | XM_011244968.2 | 90.7% | 97% | (many diffs) |
| 15 | mouse | 16597 | Klf12 | Kruppel-like factor 12 | XM_011244969.2 | 90.7% | 97% | (many diffs) |
| 16 | mouse | 16597 | Klf12 | Kruppel-like factor 12 | XM_011244970.2 | 88.8% | 94.2% | (many diffs) |
| 17 | mouse | 16597 | Klf12 | Kruppel-like factor 12 | XM_006518625.3 | 86.4% | 93.2% | (many diffs) |
| 18 | mouse | 16597 | Klf12 | Kruppel-like factor 12 | XM_011244971.2 | 86.4% | 93.2% | (many diffs) |
| 19 | mouse | 16597 | Klf12 | Kruppel-like factor 12 | XM_017315889.1 | 79.1% | 85.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1272
- ORF length:
- 1206
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tatccatatg aagagaaaaa caataaagaa tatcaacacc tttgagaaca 121 gaatgttaat gcttgatggg atgccggcag tcagagtcaa aacagagctt ttggaatctg 181 aacaagggtc tccaaacgtc cacaactatc ccgatatgga agccgttccc ctgttgctaa 241 ataatgtgaa aggggagccc ccggaggact cgttatctgt agatcacttc caaacacaaa 301 ctgagccagt ggacttgtca ataaacaaag ccaggacgtc ccctactgcc gtttcatcct 361 ccccagtttc catgacagca tctgcctcct caccttcttc aacttcaacc tcttcatcgt 421 cttctagtcg tctagcctca tccccaactg ttatcacatc agtatcttca gcgtcatctt 481 cgtcaacagt attaactcca gggccccttg tggcctctgc atctggtgtt ggaggccagc 541 agtttttgca cattatccat cccgtaccgc cttcaagtcc catgaattta cagtctaaca 601 aactgagtca tgttcaccgc atccccgtgg tggtacagtc ggtgcctgtt gtctacacag 661 ctgtaaggtc acctggaaat gtgaacaaca ctattgtcgt gccgcttttg gaggatggga 721 gaggccatgg caaagcacaa atggaccccc gaggcctatc tcccagacaa agtaaaagtg 781 acagtgatga tgatgacctg ccaaatgtga ccttagatag cgttaatgaa actGGATCTA 841 CGGCCCTTTC CATAGCCAGA GCAGTACAAG AGGTACATCC GTCCCCAGTA TCAAGGGTCC 901 GGGGAAATCG AATGAATAAT CAAAAGTTTC CTTGTTCAAT TTCACCATTT AGTATTGAGA 961 GCACAAGACG CCAGAGACGG TCTGAATCCC CAGACTCCAG AAAACGGCGT ATCCACAGAT 1021 GTGATTTTGA GGGATGCAAC AAAGTGTACA CAAAAAGTTC TCACCTGAAG GCTCACCGGA 1081 GGACACATAC AGGAGAGAAA CCTTACAAGT GTACCTGGGA AGGCTGCACC TGGAAGTTCG 1141 CTCGTTCAGA TGAACTGACG AGGCATTACC GCAAACATAC GGGAGTGAAG CCATTCAAGT 1201 GCGCGGACTG TGATCGCAGC TTTTCCCGGT CAGATCATTT GGCCCTGCAC CGCCGGAGGC 1261 ATATGTTGGT GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1321 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1381 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGAA ATTCAATCTA GGGCCCATTT 1441 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t