Construct: ORF TRCN0000470118
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009608.1_s317c1
- Derived from:
- ccsbBroadEn_14163
- DNA Barcode:
- CTTACACGCCGTATAAAGAGGGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGCR8 (54487)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470118
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54487 | DGCR8 | DGCR8 microprocessor comple... | NM_001190326.1 | 40.8% | 40.6% | 652G>A;906_906delGinsTGTT;910_2220del |
| 2 | human | 54487 | DGCR8 | DGCR8 microprocessor comple... | NM_022720.7 | 39% | 38.9% | 652G>A;906_906delGinsTGTT;910_2319del |
| 3 | human | 100302197 | MIR1306 | microRNA 1306 | NR_031706.1 | 9.3% | 0_1ins94;85_86ins733 | |
| 4 | mouse | 94223 | Dgcr8 | DGCR8, microprocessor compl... | NM_033324.2 | 36.6% | 36.2% | (many diffs) |
| 5 | mouse | 94223 | Dgcr8 | DGCR8, microprocessor compl... | XM_006522795.2 | 35.6% | 35.2% | (many diffs) |
| 6 | mouse | 94223 | Dgcr8 | DGCR8, microprocessor compl... | XM_017317176.1 | 35.6% | 35.2% | (many diffs) |
| 7 | mouse | 94223 | Dgcr8 | DGCR8, microprocessor compl... | XR_001781877.1 | 14.8% | (many diffs) | |
| 8 | mouse | 94223 | Dgcr8 | DGCR8, microprocessor compl... | XR_001781875.1 | 14.8% | (many diffs) | |
| 9 | mouse | 94223 | Dgcr8 | DGCR8, microprocessor compl... | XR_001781878.1 | 14.8% | (many diffs) | |
| 10 | mouse | 94223 | Dgcr8 | DGCR8, microprocessor compl... | XR_001781873.1 | 14.6% | (many diffs) | |
| 11 | mouse | 94223 | Dgcr8 | DGCR8, microprocessor compl... | XR_001781876.1 | 8.8% | (many diffs) | |
| 12 | mouse | 100316814 | Mir1306 | microRNA 1306 | NR_035467.2 | 8.5% | 0_1ins99;35A>G;79_80ins734 | |
| 13 | mouse | 94223 | Dgcr8 | DGCR8, microprocessor compl... | XR_001781879.1 | 8.5% | (many diffs) | |
| 14 | mouse | 94223 | Dgcr8 | DGCR8, microprocessor compl... | XR_001781874.1 | 8.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 978
- ORF length:
- 912
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gacagatgag agcccctctc cgctcccgtg tgggcccgca ggagaagcgg 121 tgatggagag ccgagctcgc cccttccaag cgctgccccg tgagcagtct ccaccacctc 181 ccctgcaaac gtccagtggt gcagaggtaa tggacgttgg ctctggtggt gatggacagt 241 ccgaactccc tgctgaggac cccttcaact tctacggagc ttctcttctc tccaaaggat 301 ccttctctaa gggccgcctc ctcatagacc cgaactgtag tggccacagc ccgcgcaccg 361 cccggcacgc acctgcggtc cggaagttct cccctgacct taagttgctt aaggatgtaa 421 agattagcgt gagctttacc gagagctgca ggagtaagga caggaaggtg ctgtacacag 481 gagcagagcg cgacgtgcgg gcggagtgcg gtctgctcct tagccctgtc agtggggacg 541 tgcatgcttg tccctttggc gggagtgttg gtgacgggGT AGGCATAGGG GGTGAGAGTG 601 CTGATAAGAA GGATGAGGAG AATGAGCTGG ATCAGGAAAA GAGAGTGGAG TATGCAGTGC 661 TCGATGAGTT AGAAGATTTT ACTGACAATT TGGAGCTAGA TGAAGAAGGA GCAGGCAGGT 721 TCACGGCTAA AGCAATCGTT CAGAGAGACA GAGTGGATGA AGAGGCCTTG AATTTCCCCT 781 ACGAGGATGA CTTTGACAAC GATGTGGATG CTCTGCTGGA AGAAGGCCTT TGTGCCCCCA 841 AAAAGAGGCG AACAGAGGAA AAATATGGCG GAGACAGCGA CCATCCGTCC GATGGAGAGA 901 CAAGTGTGCA GCCGATGATG ACCAAGATTA AAACAGTGCT CAAAAGTCGT GGCCGCCCAC 961 CTACAGAGCC TGTTCTGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1021 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1081 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACTTACAC GCCGTATAAA 1141 GAGGGCGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt