Transcript: Mouse XR_001781875.1

PREDICTED: Mus musculus DiGeorge syndrome critical region gene 8 (Dgcr8), transcript variant X3, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Dgcr8 (94223)
Length:
5731
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001781875.1
NBCI Gene record:
Dgcr8 (94223)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_001781875.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000377041 CTTGTGCACAGTCGATGTATG pLKO_005 4136 3UTR 100% 10.800 15.120 N Dgcr8 n/a
2 TRCN0000193939 CGGCTAAAGCAATCGTTCAAA pLKO.1 1023 3UTR 100% 5.625 7.875 N Dgcr8 n/a
3 TRCN0000159850 GTAAAGATTAGCGTGAGCTTT pLKO.1 716 3UTR 100% 4.950 6.930 N DGCR8 n/a
4 TRCN0000366633 CCAATGATGACCAAGATTAAA pLKO_005 1211 3UTR 100% 15.000 12.000 N Dgcr8 n/a
5 TRCN0000159003 GCTCGATGAGTTAGAAGATTT pLKO.1 958 3UTR 100% 13.200 10.560 N DGCR8 n/a
6 TRCN0000366635 TAAGGATCAGTCATAAGTTAA pLKO_005 4364 3UTR 100% 13.200 10.560 N Dgcr8 n/a
7 TRCN0000366707 TCTGGAGGAATTTCGTAATTA pLKO_005 1672 3UTR 100% 15.000 10.500 N Dgcr8 n/a
8 TRCN0000162331 CCCTGACCTTAAGTTGCTTAA pLKO.1 691 3UTR 100% 10.800 7.560 N DGCR8 n/a
9 TRCN0000377109 GAAGTAATACTTCGTTGATAG pLKO_005 4495 3UTR 100% 10.800 7.560 N Dgcr8 n/a
10 TRCN0000379244 GACAATGATGTGGACGCTTTA pLKO_005 1094 3UTR 100% 10.800 7.560 N Dgcr8 n/a
11 TRCN0000194107 GCTCTCAATGTGACTGGATAT pLKO.1 4340 3UTR 100% 10.800 7.560 N Dgcr8 n/a
12 TRCN0000193276 CCGCCCTGTTTATAATTTCTT pLKO.1 1942 3UTR 100% 5.625 3.938 N Dgcr8 n/a
13 TRCN0000159648 GACAATTTGGAGCTAGATGAA pLKO.1 983 3UTR 100% 4.950 3.465 N DGCR8 n/a
14 TRCN0000161563 GCTGTTGTCTCCATATCAGAT pLKO.1 2275 3UTR 100% 4.950 3.465 N DGCR8 n/a
15 TRCN0000194043 GCAGTGAAGATACTGAGTGTT pLKO.1 4759 3UTR 100% 4.950 2.970 N Dgcr8 n/a
16 TRCN0000415187 ACGGCTAAAGCAATCGTTCAG pLKO_005 1022 3UTR 100% 4.050 2.835 N DGCR8 n/a
17 TRCN0000160021 CCACAAAGAAAGAGTTTGTTA pLKO.1 1857 3UTR 100% 0.563 0.394 N DGCR8 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001781875.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12046 pDONR223 100% 22.2% None (many diffs) n/a
2 ccsbBroad304_12046 pLX_304 0% 22.2% V5 (many diffs) n/a
3 TRCN0000474412 AATCGGTCAATGCTCCTGCGCGTC pLX_317 29.6% 22.2% V5 (many diffs) n/a
4 ccsbBroadEn_14163 pDONR223 100% 14.8% None (many diffs) n/a
5 ccsbBroad304_14163 pLX_304 0% 14.8% V5 (many diffs) n/a
6 TRCN0000470118 CTTACACGCCGTATAAAGAGGGCG pLX_317 43.9% 14.8% V5 (many diffs) n/a
Download CSV