Construct: ORF TRCN0000470119
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003349.1_s317c1
- Derived from:
- ccsbBroadEn_11166
- DNA Barcode:
- TTAACGCCGAACCTCCTCTATGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STX5 (6811)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470119
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6811 | STX5 | syntaxin 5 | NM_001330294.2 | 100% | 100% | |
| 2 | human | 6811 | STX5 | syntaxin 5 | XM_011545224.3 | 86% | 86% | 0_1ins126 |
| 3 | human | 6811 | STX5 | syntaxin 5 | NM_003164.5 | 84.7% | 84.7% | 1_162del |
| 4 | human | 6811 | STX5 | syntaxin 5 | NM_001244666.2 | 69.1% | 70.4% | 1_162del;907_944del;963_964ins140 |
| 5 | mouse | 56389 | Stx5a | syntaxin 5A | NM_001167799.1 | 76.1% | 80.5% | (many diffs) |
| 6 | mouse | 56389 | Stx5a | syntaxin 5A | NM_019829.4 | 76.1% | 80.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 969
- ORF length:
- 903
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ctgccgggat cggacccagg agtttctgtc tgcctgcaag tcgctgcaga 121 cccgtcagaa tggaatccag acaaataagc cagctttgcg tgctgtccga caacgcagtg 181 aattcaccct catggccaag cgcattggga aagaccttag caacacattt gccaagctgg 241 agaagctgac aatcttggca aagcgcaagt ccctctttga tgataaagca gtggaaattg 301 aagagctaac atatatcatc aaacaggaca tcaatagcct caacaaacaa attgctcagc 361 tccaggattt cgtgagagcc aagggcagcc agagtggccg gcacctgcag acccactcca 421 acaccattgt ggtctccttg cagtcgaaac tggcttctat gtccaatgac ttcaaatcgg 481 ttttagaagt gaggacagag aaccTGAAGC AGCAGAGGAG CCGGAGAGAG CAGTTCTCCC 541 GGGCACCTGT GTCAGCCCTG CCCCTTGCCC CTAACCACCT GGGCGGTGGT GCTGTGGTTC 601 TGGGGGCAGA GTCCCATGCC TCCAAGGATG TCGCCATCGA CATGATGGAC TCTCGGACCA 661 GCCAGCAGCT GCAGCTCATT GACGAGCAGG ATTCCTACAT CCAGAGTCGG GCAGACACCA 721 TGCAGAACAT TGAGTCGACA ATTGTTGAGT TGGGCTCCAT CTTTCAGCAG TTGGCACACA 781 TGGTTAAGGA ACAGGAGGAA ACCATTCAGA GGATCGACGA GAACGTGCTA GGAGCCCAGC 841 TGGACGTTGA GGCCGCCCAT TCAGAGATCC TCAAGTACTT CCAGTCTGTC ACCTCCAACC 901 GGTGGCTCAT GGTCAAAATC TTCCTCATCC TCATTGTCTT CTTCATCATC TTTGTGGTCT 961 TCCTTGCTTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1021 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGCCC GTAACTTGAA AGTATTTCGA 1081 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTAACG CCGAACCTCC TCTATGACAC 1141 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt