Construct: ORF TRCN0000470120
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002184.1_s317c1
- Derived from:
- ccsbBroadEn_01498
- DNA Barcode:
- TGTCAGCACTGACAAGGCTACTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCL5 (6352)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470120
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6352 | CCL5 | C-C motif chemokine ligand 5 | NM_002985.3 | 100% | 100% | |
| 2 | human | 6352 | CCL5 | C-C motif chemokine ligand 5 | NM_001278736.2 | 59% | 41.1% | 189_270del;356_462del |
| 3 | mouse | 20304 | Ccl5 | chemokine (C-C motif) ligand 5 | NM_013653.3 | 85.3% | 80.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 142
- ORF end:
- 415
- ORF length:
- 273
- Sequence:
-
1 TCTCCTTGGA ATTTGCCCTT TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG 61 TGGTTCAAAG TTTTTTTCTT CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA 121 AGTTTGTACA AAAAAGTTGG CATGAAGGTC TCCGCGGCAG CCCTCGCTGT CATCCTCATT 181 GCTACTGCCC TCTGCGCTCC TGCATCTGCC TCCCCATATT CCTCGGACAC CACACCCTGC 241 TGCTTTGCCT ACATTGCCCG CCCACTGCCC CGTGCCCACA TCAAGGAGTA TTTCTACACC 301 AGTGGCAAGT GCTCCAACCC AGCAGTCGTC TTTGTCACCC GAAAGAACCG CCAAGTGTGT 361 GCCAACCCAG AGAAGAAATG GGTTCGGGAG TACATCAACT CTTTGGAGAT GAGCTACCCA 421 ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC 481 GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT 541 ATATCTTGTG GAAAGGACGA TGTCAGCACT GACAAGGCTA CTTAACGCGT TAAGTCgaca 601 atcaacctct ggattacaaa atttgtgaaa gatt