Construct: ORF TRCN0000470127
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005515.1_s317c1
- Derived from:
- ccsbBroadEn_00577
- DNA Barcode:
- TTAATAAGCTTCTTTGCTCCAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FOXO3 (2309)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470127
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2309 | FOXO3 | forkhead box O3 | NM_001455.4 | 100% | 100% | |
| 2 | human | 2309 | FOXO3 | forkhead box O3 | NM_201559.3 | 100% | 100% | |
| 3 | human | 2309 | FOXO3 | forkhead box O3 | XM_011535626.2 | 73.2% | 71% | (many diffs) |
| 4 | human | 2309 | FOXO3 | forkhead box O3 | XM_005266867.4 | 70.4% | 69.7% | (many diffs) |
| 5 | human | 2309 | FOXO3 | forkhead box O3 | XM_017010585.1 | 70.4% | 69.7% | (many diffs) |
| 6 | human | 2309 | FOXO3 | forkhead box O3 | XM_005266868.3 | 67.3% | 67.3% | 0_1ins660 |
| 7 | human | 2309 | FOXO3 | forkhead box O3 | XM_011535628.3 | 67.3% | 67.3% | 0_1ins660 |
| 8 | human | 2309 | FOXO3 | forkhead box O3 | XM_011535629.2 | 67.3% | 67.3% | 0_1ins660 |
| 9 | human | 2309 | FOXO3 | forkhead box O3 | XM_017010586.1 | 67.3% | 67.3% | 0_1ins660 |
| 10 | human | 2310 | FOXO3B | forkhead box O3B | NM_001368134.1 | 26.7% | 26.2% | (many diffs) |
| 11 | human | 2310 | FOXO3B | forkhead box O3B | NM_001368135.1 | 26.7% | 26.2% | (many diffs) |
| 12 | mouse | 56484 | Foxo3 | forkhead box O3 | NM_019740.2 | 90.3% | 94.6% | (many diffs) |
| 13 | mouse | 56484 | Foxo3 | forkhead box O3 | XM_006512806.1 | 90.3% | 94.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2085
- ORF length:
- 2019
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agaggcaccg gcttccccgg ccccgctctc tccgctcgaa gtggagctgg 121 acccggagtt cgagccccag agccgtccgc gatcctgtac gtggcccctg caaaggccgg 181 agctccaagc gagccctgcc aagccctcgg gggagacggc cgccgactcc atgatccccg 241 aggaggagga cgatgaagac gacgaggacg gcgggggacg ggccggctcg gccatggcga 301 tcggcggcgg cggcgggagc ggcacgctgg gctccgggct gctccttgag gactcggccc 361 gggtgctggc acccggaggg caagaccccg ggtctgggcc agccaccgcg gcgggcgggc 421 tgagcggggg tacacaggcg ctgctgcagc ctcagcaacc gctgccaccg ccgcagccgg 481 gggcggctgg gggctccggg cagccgagga aatgttcgtc gcggcggaac gcctggggaa 541 acctgtccta cgcggacctg atcacccgcg ccatcgagag ctccccggac aaacggctca 601 ctctgtccca gatctacgag tggatggtgc gttgcgtgcc ctacttcaag gataagggcg 661 acagcaacag ctctgccggc tggaagaact ccatccggca caacctgtca ctgcatagtc 721 gattcatgcg ggtccagaat gagggaactg gcaagagctc ttggtggatc atcaaccctg 781 atggggggaa gagcggaaaa gccccccggc ggcgggctgt ctccatggac aatagcaaca 841 agtataccaa gagccgtggc cgcgcagcca agaagaaggc agccctgcag acagcccccg 901 aatcagctga cgacagtccc tcccagctct ccaagtggcc tggcagcccc acgtcacgca 961 gcagtgatga gctggatgcg tggacggact tccgttcacg caccaattct aacgccagca 1021 cagtcagtgg ccgcctgtcg cccatcatgg caagcacaga gttggatgaa gtccaggacg 1081 atgatgcgcc tctctcgccc atgctctaca gcagctcagc cagcctgtca ccttcagtaa 1141 gcaagccgtg cacggtggaa ctgccacggc tgactgatat ggcaggcacc atgaatctga 1201 atgatgggct gactgaaaac ctcatggacg acctgctgga taacatcacg ctcccgccat 1261 cccagccatc gcccactggg ggactcatgc agcggagctc tagcttcccg tataccacca 1321 agggctcggg cctgggctcc ccaaccagct cctttaacag cacggtgttc ggaccttcat 1381 ctctgaactc cctacgccag tctcccatgc agaccatcca agagaacaag ccagctacct 1441 tctcttccat gtcacactat ggtaaccaga cactccagga cctgctcact tcggactcac 1501 ttagccacag cgatgtcatg atgacacagt cggacccctt gatgtctcag gccagcaccg 1561 ctgtgtctgc ccagaattcc cgccggaacg tgatgcttcg caatgatccg atgatgtcct 1621 ttgctgccca gcctaaccag ggaagtttgg tcaatcagaa cttgctccac caccagcacc 1681 aaacccaggg cgctcttggt ggcagccgtg ccttgtcgaa tTCTGTCAGC AACATGGGCT 1741 TGAGTGAGTC CAGCAGCCTT GGGTCAGCCA AACACCAGCA GCAGTCTCCT GTCAGCCAGT 1801 CTATGCAAAC CCTCTCGGAC TCTCTCTCAG GCTCCTCCTT GTACTCAACT AGTGCAAACC 1861 TGCCCGTCAT GGGCCATGAG AAGTTCCCCA GCGACTTGGA CCTGGACATG TTCAATGGGA 1921 GCTTGGAATG TGACATGGAG TCCATTATCC GTAGTGAACT CATGGATGCT GATGGGTTGG 1981 ATTTTAACTT TGATTCCCTC ATCTCCACAC AGAATGTTGT TGGTTTGAAC GTGGGGAACT 2041 TCACTGGTGC TAAGCAGGCC TCATCTCAGA GCTGGGTGCC AGGCTGCCCA ACTTTCTTGT 2101 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2161 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2221 GAAAGGACGA TTAATAAGCT TCTTTGCTCC AATAACGCGT TAAGTCgaca atcaacctct 2281 ggattacaaa atttgtgaaa gatt