Construct: ORF TRCN0000470137
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004779.1_s317c1
- Derived from:
- ccsbBroadEn_15983
- DNA Barcode:
- CTTAAATGCTCTCTACTCTTACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM43 (79188)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470137
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79188 | TMEM43 | transmembrane protein 43 | NM_024334.2 | 99.7% | 99.2% | 504A>T;536T>C;1013C>T |
| 2 | human | 79188 | TMEM43 | transmembrane protein 43 | XM_017007176.2 | 91% | 90.5% | (many diffs) |
| 3 | mouse | 74122 | Tmem43 | transmembrane protein 43 | NM_028766.3 | 86.5% | 92.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1266
- ORF length:
- 1200
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cgcgaattat tccagtacca gtacccggag agaacatgtc aaagttaaaa 121 ccagctccca gccaggcttc ctggaacggc tgagcgagac ctcgggtggg atgtttgtgg 181 ggctcatggc cttcctgctc tccttctacc taattttcac caatgagggc cgcgcattga 241 agacggcaac ctcattggct gaggggctct cgcttgtggt gtctcccgac agcatccaca 301 gtgtggctcc ggagaatgaa ggaaggctgg tgcacatcat tggcgcctta cggacatcca 361 agcttttgtc tgatccaaac tatggggtcc atcttccggc tgtgaaactg cggaggcacg 421 tggagatgta ccaatgggta gaaactgagg agtccaggga gtacaccgag gatgggcagg 481 tgaagaagga gacgaggtat tcctacaaca ctgaatggag gtcagaaatc atcaacagca 541 aaaacttcga ccgagagatt ggccacaata accccagtgc catggcagtg gagtcattca 601 cggcaacagc cccctttgtc caaattggca ggtttttcct ctcgtcaggc ctcatcgaca 661 aagtcgacaa cttcaagtcc ctgagcctat ccaagctgga ggaccctcat gtggacatca 721 ttcgccgtgg agactttttc taccacagcg aaaatcccaa gtatccagag gtgggagact 781 tgcgtgtctc cttttcctat gctggactga gcggcgatga ccctgacctg ggcccagctc 841 acgtggtcac tgtgattgcc cggcagcggg gtgaccagct agtcccattc tccaccaagt 901 ctggggatac cTTACTGCTC CTGCACCACG GGGACTTCTC AGCAGAGGAG GTGTTTCATA 961 GAGAACTAAG GAGCAACTCC ATGAAGACCT GGGGCCTGCG GGCAGCTGGC TGGATGGCCA 1021 TGTTCATGGG CCTCAACCTT ATGACACGGA TCCTCTACAC CTTGGTGGAC TGGTTTCTTG 1081 TTTTCCGAGA CCTGGTCAAC ATTGGCCTGA AAGCCTTTGC CTTCTGTGTG GCCACCTCGC 1141 TGACCCTGCT GACCGTGGCG GCTGGCTGGC TCTTCTACCG ACCCCTGTGG GCCCTCCTCA 1201 TTGCCGGCCT GGCCCTTGTG CCCATCCTTG TTGCTCGGAC ACGGGTGCCA GCCAAAAAGT 1261 TGGAGTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1321 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1381 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACTTAAATGC TCTCTACTCT TACAGACGCG 1441 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt