Construct: ORF TRCN0000470155
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012892.1_s317c1
- Derived from:
- ccsbBroadEn_10764
- DNA Barcode:
- CTTTGGGGGGCTTGTCAAAATAGA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP11B1 (1584)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470155
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1584 | CYP11B1 | cytochrome P450 family 11 s... | NM_000497.3 | 84.2% | 84.2% | (many diffs) |
| 2 | human | 1585 | CYP11B2 | cytochrome P450 family 11 s... | NM_000498.3 | 80.3% | 78.5% | (many diffs) |
| 3 | human | 1584 | CYP11B1 | cytochrome P450 family 11 s... | NM_001026213.1 | 73.1% | 73.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1860
- ORF length:
- 1791
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcactcagg gcaaaggcag aggtgtgcat ggcagtgccc tggctgtccc 121 tgcaaagggc acaggcactg ggcacgagag ccgcccgggt ccccaggaca gtgctgccct 181 ttgaagccat gccccggcgt ccaggcaaca ggtggctgag gctgctgcag atctggaggg 241 agcagggtta tgaggacctg cacctggaag tacaccagac cttccaggaa ctggggccca 301 ttttcaggtc ccggcactct gcatcctttg gaagatgggg aaggagtgca gcacgtgctg 361 gtctgtggcg ctgccagggc aggggatggt gcagagcaaa tcccagctcg ctgcagagag 421 ggcaggactc agaggcactg aagtacgatt tgggaggagc aggcatggtg tgtgtgatgc 481 tgccggagga cgtggagaag ctgcaacagg tggacagcct gcatccccac aggatgagcc 541 tggagccctg ggtggcctac agacaacatc gtgggcacaa atgtggcgtg ttcttgctga 601 atgtggctga caggggaaat tcctccccac cattccctgg gggcatccat ggagccccca 661 cgcactctgg ctgtaggaat gggcctgaat ggcgcttcaa ccgattgcgg ctgaatccag 721 aagtgctgtc gcccaacgct gtgcagaggt tcctcccgat ggtggatgca gtggccaggg 781 acttctccca ggccctgaag aagaaggtgc tgcagaacgc ccgggggagc ctgaccctgg 841 acgtccagcc cagcatcttc cactacacca tagaagccag caacttggct ctttttggag 901 agcggctggg cctggttggc cacagcccca gttctgccag cctgaacttc ctccatgccc 961 tggaggtcat gttcaaatcc accgtccagc tcatgttcat gcccaggagc ctgtctcgct 1021 ggaccagccc caaggtgtgg aaggagcact ttgaggcctg ggactgcatc ttccagtacg 1081 gcgacaactg tatccagaaa atctatcagg aactggcctt cagccgccct caacagtaca 1141 ccagcatcgt ggcggagctc ctgttgaatg cggaactgtc gccagatgcc atcaaggcca 1201 actctatgga actcactgca gggagcgtgg acacgacggt gtttcccttg ctgatgacgc 1261 tctttgagct ggctcggaac cccaacgtgc agcaggccct gcgccaggag agcctggccg 1321 ccgcagccag catcagtgaa catccccaga aggcaaccac cgagctgccc ttgctgcgtg 1381 cggccctcaa ggagaccttg cggctctacc ctgtgggtct gtttctggag cgagtggcga 1441 gctcagactt GGTGCTTCAG AACTACCACA TCCCAGCTGG GACATTGGTG CGCGTGTTCC 1501 TCTACTCTCT GGGTCGCAAC CCCGCCTTGT TCCCGAGGCC TGAGCGCTAT AACCCCCAGC 1561 GCTGGCTAGA CATCAGGGGC TCCGGCAGGA ACTTCTACCA CGTGCCCTTT GGCTTTGGCA 1621 TGCGCCAGTG CCTTGGGCGG CGCCTGGCAG AGGCAGAGAT GCTGCTGCTG CTGCACCATG 1681 TGCTGAAACA CCTCCAGGTG GAGACACTAA CCCAAGAGGA CATAAAGATG GTCTACAGCT 1741 TCATATTGAG GCCCAGCATG TTCCCCCTCC TCACCTTCAG AGCCATCAAC AAAGATGGTC 1801 TACAGCTTCA TATTGAGGCC CAGCATGTTC CCCCTCCTCA CCTTCAGAGC CATCAACTTG 1861 CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT 1921 CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT 1981 TATATATCTT GTGGAAAGGA CGACTTTGGG GGGCTTGTCA AAATAGAACG CGTTAAGTCg 2041 acaatcaacc tctggattac aaaatttgtg aaagatt