Construct: ORF TRCN0000470161
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005575.1_s317c1
- Derived from:
- ccsbBroadEn_10838
- DNA Barcode:
- AAGAAGACCAACCGCAGCCACAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAS2 (2620)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470161
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2620 | GAS2 | growth arrest specific 2 | NM_001351224.2 | 57.6% | 56.3% | (many diffs) |
| 2 | human | 2620 | GAS2 | growth arrest specific 2 | XM_011519975.1 | 56.6% | 55.4% | (many diffs) |
| 3 | human | 2620 | GAS2 | growth arrest specific 2 | XM_017017532.1 | 50% | 48.9% | (many diffs) |
| 4 | human | 2620 | GAS2 | growth arrest specific 2 | NM_001143830.2 | 44.7% | 43.7% | (many diffs) |
| 5 | human | 2620 | GAS2 | growth arrest specific 2 | NM_005256.4 | 44.7% | 43.7% | (many diffs) |
| 6 | human | 2620 | GAS2 | growth arrest specific 2 | NM_177553.3 | 44.7% | 43.7% | (many diffs) |
| 7 | human | 2620 | GAS2 | growth arrest specific 2 | XM_011519971.3 | 42.2% | 41.3% | (many diffs) |
| 8 | human | 2620 | GAS2 | growth arrest specific 2 | XM_011519972.3 | 42.2% | 41.3% | (many diffs) |
| 9 | human | 2620 | GAS2 | growth arrest specific 2 | XR_001747829.1 | 19.4% | (many diffs) | |
| 10 | human | 2620 | GAS2 | growth arrest specific 2 | NR_147085.2 | 15.5% | (many diffs) | |
| 11 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_017321982.1 | 41.5% | 43.2% | (many diffs) |
| 12 | mouse | 14453 | Gas2 | growth arrest specific 2 | NM_001330607.1 | 40% | 42% | (many diffs) |
| 13 | mouse | 14453 | Gas2 | growth arrest specific 2 | NM_008087.3 | 40% | 42% | (many diffs) |
| 14 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_006540624.3 | 40% | 42% | (many diffs) |
| 15 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_006540625.3 | 40% | 42% | (many diffs) |
| 16 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_006540626.3 | 40% | 42% | (many diffs) |
| 17 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_006540618.3 | 37.1% | 38.7% | (many diffs) |
| 18 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_006540619.3 | 37.1% | 38.7% | (many diffs) |
| 19 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_006540620.3 | 37.1% | 38.7% | (many diffs) |
| 20 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_006540621.3 | 37.1% | 38.7% | (many diffs) |
| 21 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_006540622.3 | 37.1% | 38.7% | (many diffs) |
| 22 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_006540623.3 | 37.1% | 38.7% | (many diffs) |
| 23 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_011250796.2 | 37.1% | 38.7% | (many diffs) |
| 24 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_011250797.2 | 37.1% | 38.7% | (many diffs) |
| 25 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_017321979.1 | 37.1% | 38.7% | (many diffs) |
| 26 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_017321980.1 | 37.1% | 38.7% | (many diffs) |
| 27 | mouse | 14453 | Gas2 | growth arrest specific 2 | XM_017321981.1 | 37.1% | 38.7% | (many diffs) |
| 28 | mouse | 14453 | Gas2 | growth arrest specific 2 | NM_001330608.1 | 23.7% | 23.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 489
- ORF length:
- 423
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtg cactgcTCTG AGCCCAAAGG TACGCAGTGG GCCTGGCCTC TCTGATATGC 121 ATCAGTATAG CCAATGGCTA GCCAGCAGAC ATGAAGCTAA TTTGCTACCA ATGAAAGAAG 181 ATCTGGCCTT GTGGTTAACC AATCTATTAG GGAAGGAGAT TACAGCAGAA ACTTTTATGG 241 AGAAGTTGGA CAATGGTGCC TTGCTCTGTC AACTTGCAGA AACTATGCAG GAGAAATTCA 301 AGGAGAGCAT GGATGCTAAC AAGCCCACAA AGAATCTACC GTTGAAGAAG ATCCCATGCA 361 AAACCAGTGC ACCCTCGGGC TCCTTTTTTG CCAGAGACAA TACAGCAAAT TTCTTATCCT 421 GGTGCCGAGA TTTAGGGGTG GATGAAACGT GTCTATTTGA ATCGGAAGGT TTGGGATTTG 481 GAGGCCAGTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAGAAG ACCAACCGCA GCCACAGCAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt