Construct: ORF TRCN0000470162
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008415.1_s317c1
- Derived from:
- ccsbBroadEn_00001
- DNA Barcode:
- TGCCCTGTAAGGCGGGTTATTTCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NAT1 (9)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470162
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9 | NAT1 | N-acetyltransferase 1 | NM_000662.8 | 100% | 100% | |
| 2 | human | 9 | NAT1 | N-acetyltransferase 1 | NM_001160170.4 | 100% | 100% | |
| 3 | human | 9 | NAT1 | N-acetyltransferase 1 | NM_001160171.4 | 100% | 100% | |
| 4 | human | 9 | NAT1 | N-acetyltransferase 1 | NM_001160172.4 | 100% | 100% | |
| 5 | human | 9 | NAT1 | N-acetyltransferase 1 | NM_001160173.3 | 100% | 100% | |
| 6 | human | 9 | NAT1 | N-acetyltransferase 1 | NM_001160174.2 | 100% | 100% | |
| 7 | human | 9 | NAT1 | N-acetyltransferase 1 | NM_001160179.3 | 100% | 100% | |
| 8 | human | 9 | NAT1 | N-acetyltransferase 1 | XM_006716410.3 | 100% | 100% | |
| 9 | human | 9 | NAT1 | N-acetyltransferase 1 | XM_011544689.2 | 100% | 100% | |
| 10 | human | 10 | NAT2 | N-acetyltransferase 2 | NM_000015.3 | 86.6% | 80.6% | (many diffs) |
| 11 | human | 10 | NAT2 | N-acetyltransferase 2 | XM_017012938.1 | 86.6% | 80.6% | (many diffs) |
| 12 | human | 9 | NAT1 | N-acetyltransferase 1 | NM_001160175.4 | 82.3% | 82.3% | 1_186del |
| 13 | human | 9 | NAT1 | N-acetyltransferase 1 | NM_001160176.4 | 82.3% | 82.3% | 1_186del |
| 14 | human | 9 | NAT1 | N-acetyltransferase 1 | NM_001291962.2 | 82.3% | 82.3% | 1_186del |
| 15 | human | 9 | NAT1 | N-acetyltransferase 1 | XM_011544687.1 | 82.3% | 82.3% | 1_186del |
| 16 | human | 9 | NAT1 | N-acetyltransferase 1 | XM_011544688.1 | 82.3% | 82.3% | 1_186del |
| 17 | human | 9 | NAT1 | N-acetyltransferase 1 | XM_017013947.1 | 82.3% | 82.3% | 1_186del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 936
- ORF length:
- 870
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cattgaagca tatcttgaaa gaattggcta taagaagtct aggaacaaat 121 tggacttgga aacattaact gacattcttc aacaccagat ccgagctgtt ccctttgaga 181 accttaacat ccattgtggg gatgccatgg acttaggctt agaggccatt tttgatcaag 241 ttgtgagaag aaatcggggt ggatggtgtc tccaggtcaa tcatcttctg tactgggctc 301 tgaccactat tggttttgag accacgatgt tgggagggta tgtttacagc actccagcca 361 aaaaatacag cactggcatg attcaccttc tcctgcaggt gaccattgat ggcaggaact 421 acattgtcga tgctgggttt ggacgctcat accagatgtg gcagcctctg gagttaattt 481 ctgggaagga tcagcctcag gtgccttgtg tcttccgttT GACGGAAGAG AATGGATTCT 541 GGTATCTAGA CCAAATCAGA AGGGAACAGT ACATTCCAAA TGAAGAATTT CTTCATTCTG 601 ATCTCCTAGA AGACAGCAAA TACCGAAAAA TCTACTCCTT TACTCTTAAG CCTCGAACAA 661 TTGAAGATTT TGAGTCTATG AATACATACC TGCAGACATC TCCATCATCT GTGTTTACTA 721 GTAAATCATT TTGTTCCTTG CAGACCCCAG ATGGGGTTCA CTGTTTGGTG GGCTTCACCC 781 TCACCCATAG GAGATTCAAT TATAAGGACA ATACAGATCT AATAGAGTTC AAGACTCTGA 841 GTGAGGAAGA AATAGAAAAA GTGCTGAAAA ATATATTTAA TATTTCCTTG CAGAGAAAGC 901 TTGTGCCCAA ACATGGTGAT AGATTTTTTA CTATTTACCC AACTTTCTTG TACAAAGTGG 961 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1021 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1081 ATGCCCTGTA AGGCGGGTTA TTTCAACGCG TTAAGTCgac aatcaacctc tggattacaa 1141 aatttgtgaa agatt