Construct: ORF TRCN0000470166
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017309.1_s317c1
- Derived from:
- ccsbBroadEn_01867
- DNA Barcode:
- CCCCCACCTCTATGAGGGGTTTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GAN (8139)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470166
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8139 | GAN | gigaxonin | NM_022041.3 | 100% | 100% | |
| 2 | human | 8139 | GAN | gigaxonin | XM_017023734.1 | 64.3% | 64.3% | 0_1ins639 |
| 3 | mouse | 209239 | Gan | giant axonal neuropathy | NM_001081151.1 | 87.5% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1857
- ORF length:
- 1791
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgagggcagt gccgtgtctg accctcagca cgccgcgcgt ctgctgcgag 121 cgctcagctc tttccgcgag gagtctcgct tctgcgacgc gcacctggtc ctcgacgggg 181 aggagatccc ggtgcagaag aacatcctgg cggcggccag cccgtacatc aggacaaagt 241 taaactataa tcctccaaaa gatgatggat caacttataa gattgaactt gaagggatat 301 cggtaatggt tatgagagag atcctggatt acatcttcag tgggcagatc aggctaaatg 361 aagatacaat ccaagatgtt gttcaggcag ctgacctgct gctactgacg gaccttaaaa 421 ccctgtgctg tgagtttttg gaaggctgca ttgctgctga gaactgtatt ggtatccgtg 481 actttgcact acattactgc ctccatcacg ttcattacct tgccacagaa tacctggaga 541 ctcatttccg agacgtcagc agcacggaag aattcttaga gctgagtcct caaaagctta 601 aagaagtgat ttctcttgag aagttaaacg ttggcaatga aagatatgtc tttgaagcag 661 taattcgatg gatagcacat gatacagaaa taagaaaggt ccacatgaag gatgttatgt 721 cagctctgtg ggtttcaggg ttggactcca gttatttacg ggaacagatg ctgaatgaac 781 cattagtacg agaaattgtc aaagagtgta gcaatatacc gctcagccag ccgcagcaag 841 gggaggcgat gctggccaac ttcaaacccc ggggctactc tgagtgcatc gtgactgttg 901 gtggagaaga gagagtttca cggaaaccca cagcagcgat gcgatgcatg tgccctctct 961 atgaccctaa caggcagctt tggatcgaac tggccccttt aagcatgccg agaattaacc 1021 atggagttct ctcagcagaa ggatttttgt ttgtattcgg gggccaagat gaaaataagc 1081 agactcttag ctcaggagaa aagtatgatc cagatgcaaa tacatggaca gcattgccac 1141 ctatgaacga ggcaagacat aacttcggaa ttgtggagat agatgggatg ctgtacattt 1201 tgggaggaga ggatggtgaa aaggagctga tttccatgga gtgttacgat atttattcta 1261 aaacctggac aaagcaacct gatttgacca tggtcagaaa gatcggctgc tatgcagcta 1321 tgaaaaagaa aatctacgcc atgggtggag gctcctacgg aaagcttttt gagtctgtag 1381 agtgttatga tcccaggacc cagcagtgga ctgccatatg tccactaaaa gagaggaggt 1441 ttGGAGCGGT GGCCTGTGGA GTTGCTATGG AGCTGTATGT GTTTGGGGGA GTCCGAAGTC 1501 GTGAGGACGC CCAGGGTAGC GAGATGGTAA CTTGCAAGTC CGAGTTCTAC CATGATGAGT 1561 TTAAAAGGTG GATCTATCTT AACGACCAGA ATTTATGCAT CCCCGCCAGT TCCTCTTTTG 1621 TTTATGGAGC TGTACCTATA GGAGCCAGTA TTTATGTTAT TGGAGATCTT GATACAGGTA 1681 CCAATTACGA CTACGTGCGT GAGTTTAAAA GAAGCACAGG AACCTGGCAC CACACTAAAC 1741 CACTCCTTCC ATCCGACCTT CGCCGTACAG GATGTGCAGC CTTACGCATT GCGAATTGCA 1801 AGCTTTTCCG CCTGCAGCTT CAGCAAGGCT TATTCCGTAT TCGTGTTCAT TCCCCTTGCC 1861 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1921 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1981 ATATATCTTG TGGAAAGGAC GACCCCCACC TCTATGAGGG GTTTACACGC GTTAAGTCga 2041 caatcaacct ctggattaca aaatttgtga aagatt