Construct: ORF TRCN0000470166

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017309.1_s317c1
Derived from:
ccsbBroadEn_01867
DNA Barcode:
CCCCCACCTCTATGAGGGGTTTAC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GAN (8139)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470166
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 8139 GAN gigaxonin NM_022041.3 100% 100%
2 human 8139 GAN gigaxonin XM_017023734.1 64.3% 64.3% 0_1ins639
3 mouse 209239 Gan giant axonal neuropathy NM_001081151.1 87.5% 98.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1857
ORF length:
1791
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgagggcagt gccgtgtctg accctcagca cgccgcgcgt ctgctgcgag
121cgctcagctc tttccgcgag gagtctcgct tctgcgacgc gcacctggtc ctcgacgggg
181aggagatccc ggtgcagaag aacatcctgg cggcggccag cccgtacatc aggacaaagt
241taaactataa tcctccaaaa gatgatggat caacttataa gattgaactt gaagggatat
301cggtaatggt tatgagagag atcctggatt acatcttcag tgggcagatc aggctaaatg
361aagatacaat ccaagatgtt gttcaggcag ctgacctgct gctactgacg gaccttaaaa
421ccctgtgctg tgagtttttg gaaggctgca ttgctgctga gaactgtatt ggtatccgtg
481actttgcact acattactgc ctccatcacg ttcattacct tgccacagaa tacctggaga
541ctcatttccg agacgtcagc agcacggaag aattcttaga gctgagtcct caaaagctta
601aagaagtgat ttctcttgag aagttaaacg ttggcaatga aagatatgtc tttgaagcag
661taattcgatg gatagcacat gatacagaaa taagaaaggt ccacatgaag gatgttatgt
721cagctctgtg ggtttcaggg ttggactcca gttatttacg ggaacagatg ctgaatgaac
781cattagtacg agaaattgtc aaagagtgta gcaatatacc gctcagccag ccgcagcaag
841gggaggcgat gctggccaac ttcaaacccc ggggctactc tgagtgcatc gtgactgttg
901gtggagaaga gagagtttca cggaaaccca cagcagcgat gcgatgcatg tgccctctct
961atgaccctaa caggcagctt tggatcgaac tggccccttt aagcatgccg agaattaacc
1021atggagttct ctcagcagaa ggatttttgt ttgtattcgg gggccaagat gaaaataagc
1081agactcttag ctcaggagaa aagtatgatc cagatgcaaa tacatggaca gcattgccac
1141ctatgaacga ggcaagacat aacttcggaa ttgtggagat agatgggatg ctgtacattt
1201tgggaggaga ggatggtgaa aaggagctga tttccatgga gtgttacgat atttattcta
1261aaacctggac aaagcaacct gatttgacca tggtcagaaa gatcggctgc tatgcagcta
1321tgaaaaagaa aatctacgcc atgggtggag gctcctacgg aaagcttttt gagtctgtag
1381agtgttatga tcccaggacc cagcagtgga ctgccatatg tccactaaaa gagaggaggt
1441ttGGAGCGGT GGCCTGTGGA GTTGCTATGG AGCTGTATGT GTTTGGGGGA GTCCGAAGTC
1501GTGAGGACGC CCAGGGTAGC GAGATGGTAA CTTGCAAGTC CGAGTTCTAC CATGATGAGT
1561TTAAAAGGTG GATCTATCTT AACGACCAGA ATTTATGCAT CCCCGCCAGT TCCTCTTTTG
1621TTTATGGAGC TGTACCTATA GGAGCCAGTA TTTATGTTAT TGGAGATCTT GATACAGGTA
1681CCAATTACGA CTACGTGCGT GAGTTTAAAA GAAGCACAGG AACCTGGCAC CACACTAAAC
1741CACTCCTTCC ATCCGACCTT CGCCGTACAG GATGTGCAGC CTTACGCATT GCGAATTGCA
1801AGCTTTTCCG CCTGCAGCTT CAGCAAGGCT TATTCCGTAT TCGTGTTCAT TCCCCTTGCC
1861CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1921TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1981ATATATCTTG TGGAAAGGAC GACCCCCACC TCTATGAGGG GTTTACACGC GTTAAGTCga
2041caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)