Construct: ORF TRCN0000470171
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005225.1_s317c1
- Derived from:
- ccsbBroadEn_07331
- DNA Barcode:
- GCGTCGTGGCATAACCTCCCTACT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOP3B (8940)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470171
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8940 | TOP3B | DNA topoisomerase III beta | NM_001282112.2 | 99.8% | 99.6% | 960C>T;1093G>A;2581delT |
| 2 | human | 8940 | TOP3B | DNA topoisomerase III beta | NM_001282113.2 | 99.8% | 99.6% | 960C>T;1093G>A;2581delT |
| 3 | human | 8940 | TOP3B | DNA topoisomerase III beta | NM_001349845.2 | 99.8% | 99.6% | 960C>T;1093G>A;2581delT |
| 4 | human | 8940 | TOP3B | DNA topoisomerase III beta | NM_001349847.2 | 99.8% | 99.6% | 960C>T;1093G>A;2581delT |
| 5 | human | 8940 | TOP3B | DNA topoisomerase III beta | NM_003935.5 | 99.8% | 99.6% | 960C>T;1093G>A;2581delT |
| 6 | human | 8940 | TOP3B | DNA topoisomerase III beta | NM_001349848.2 | 84.1% | 83.8% | (many diffs) |
| 7 | human | 8940 | TOP3B | DNA topoisomerase III beta | NM_001349850.2 | 84.1% | 83.8% | (many diffs) |
| 8 | human | 8940 | TOP3B | DNA topoisomerase III beta | NM_001349851.2 | 66.9% | 65.9% | (many diffs) |
| 9 | human | 8940 | TOP3B | DNA topoisomerase III beta | NM_001349852.2 | 66.9% | 65.9% | (many diffs) |
| 10 | human | 8940 | TOP3B | DNA topoisomerase III beta | NR_146277.2 | 66% | (many diffs) | |
| 11 | mouse | 21976 | Top3b | topoisomerase (DNA) III beta | NM_001326576.1 | 86.8% | 95.2% | (many diffs) |
| 12 | mouse | 21976 | Top3b | topoisomerase (DNA) III beta | NM_011624.3 | 86.8% | 95.2% | (many diffs) |
| 13 | mouse | 21976 | Top3b | topoisomerase (DNA) III beta | XM_006521991.3 | 86.8% | 95.2% | (many diffs) |
| 14 | mouse | 21976 | Top3b | topoisomerase (DNA) III beta | XM_006521992.3 | 86.8% | 95.2% | (many diffs) |
| 15 | mouse | 21976 | Top3b | topoisomerase (DNA) III beta | XM_006521994.3 | 86.8% | 95.2% | (many diffs) |
| 16 | mouse | 21976 | Top3b | topoisomerase (DNA) III beta | XM_006521995.2 | 86.8% | 95.2% | (many diffs) |
| 17 | mouse | 21976 | Top3b | topoisomerase (DNA) III beta | XM_017316937.1 | 86.8% | 95.2% | (many diffs) |
| 18 | mouse | 21976 | Top3b | topoisomerase (DNA) III beta | XM_006521997.3 | 72.9% | 77% | (many diffs) |
| 19 | mouse | 21976 | Top3b | topoisomerase (DNA) III beta | XR_001781810.1 | 71.6% | (many diffs) | |
| 20 | mouse | 21976 | Top3b | topoisomerase (DNA) III beta | XR_384545.3 | 61.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2652
- ORF length:
- 2586
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gactgtgctc atggttgctg aaaagccgtc cttggcacag tcaattgcca 121 aaatcctctc tagagggagc ctgtcctcac acaaagggct gaacggggcc tgctcagtcc 181 acgagtacac tgggaccttt gctggccagc cagtgcgctt caagatgacg tctgtctgtg 241 gtcacgtgat gaccctggat ttcctgggaa aatacaacaa atgggacaaa gtggaccccg 301 cagaactgtt cagccaagct cccacggaga agaaagaagc taaccccaag ctgaacatgg 361 tgaagttcct gcaggtggag ggcagaggct gcgactacat cgtgctgtgg ctggactgcg 421 acaaggaggg ggagaacatc tgctttgagg ttcttgatgc tgttctgccc gtcatgaaca 481 aggcccatgg tggcgagaag accgtgttcc gggccaggtt tagctccatc acggacacag 541 acatctgtaa tgccatggcc tgcctaggcg agcctgacca caacgaggcg ctctcagtgg 601 atgctcgcca ggagctggac ctgcgaatcg gctgtgcatt caccaggttt cagactaaat 661 atttccaggg gaaatacggt gatttagaca gctctctcat ctcctttggg ccgtgtcaga 721 ctccaaccct gggattctgt gtggagagac atgataaaat ccagtccttc aaaccagaga 781 cctactgggt gctgcaggcc aaggttaaca ctgacaaaga cagatctctc cttttggact 841 gggaccgagt aagagtgttt gaccgggaga tcgcacagat gtttttaaac atgacaaagc 901 tggagaagga agcccaggtg gaggccacaa gcaggaaaga aaaggccaag cagaggcccc 961 tggccctgaa cactgtggag atgctgcgtg tggccagctc ttctctgggc atggggccgc 1021 agcatgccat gcagacggct gagcggctct acacgcaagg ctacatcagc tacccacgga 1081 cagagaccac ccactaccct gagaactttg acctgaaggg ctctctgcgg cagcaggcca 1141 accaccccta ctgggccaac acggtgaagc ggttgttagc agaaggtatc aaccgcccgc 1201 ggaaaggcca tgacgccggc gaccatcccc ccatcacccc catgaagtct gccacagagg 1261 ccgaattagg gggtgacgcg tggcggctct atgagtacat caccagacac ttcatcgcca 1321 cggtcagcca tgactgcaag tacctgcaga gcaccatctc cttcagaatt gggcccgagc 1381 tcttcacctg ctccgggaag accgtcctct caccaggctt cacggaggtc atgccctggc 1441 agagcgtgcc cctggaggag agcctgccca cttgccagcg gggtgatgcc ttccctgtgg 1501 gcgaggtgaa gatgctggag aagcagacga acccacccga ctacctgacg gaggccgagc 1561 tcatcacgct catggagaag catggcatcg gcacggatgc cagcatccct gtgcatatca 1621 acaacatctg ccagcgcaac tatgtcacgg tggagagcgg gcgccggctc aagcccacca 1681 acctcggcat cgtcctggtg cacggctact ataagattga tgcagagctg gtgctcccca 1741 ccatccgcag tgcagtggag aagcagctga acctgatcgc ccagggcaag gccgactacc 1801 gccaggtcct gggccacacc ctggacgtgt tcaagaggaa gttccactac tttgtcgact 1861 ccattgctgg catggatgag ttgatggagg tgtctttctc gcccctggcg gccacaggca 1921 agcccctctc acgctgtggg aagtgccacc gcttcatgaa gtacatccag gccaagccaa 1981 gccgcctgca ctgctcccac tgcgatgaga cctacacgct cccccagaac ggcaccatca 2041 agctctacaa ggagctccgc tgccctctgg atgacttcga gctggtcctg tggtcatcag 2101 gctctcgggg caagagctac ccgctgtgcc cctactgcta caaccaccca cccttccgag 2161 acatgaagaa aggcatgggc tgcaacgagt gtacgcaccc cTCCTGCCAG CACTCGCTGA 2221 GCATGCTGGG CATCGGCCAG TGCGTGGAAT GTGAGAGCGG GGTGCTGGTG CTGGACCCCA 2281 CCTCGGGCCC CAAGTGGAAG GTGGCCTGCA ACAAGTGCAA CGTGGTAGCG CACTGCTTCG 2341 AGAACGCCCA CCGCGTGCGG GTGTCCGCCG ACACCTGCAG TGTCTGTGAG GCCGCCTTGC 2401 TTGATGTGGA CTTCAACAAG GCCAAGTCCC CACTCCCGGG CGATGAGACG CAGCACATGG 2461 GCTGCGTCTT TTGTGACCCC GTCTTCCAGG AGCTGGTGGA GCTGAAGCAT GCGGCCTCCT 2521 GCCACCCCAT GCACCGCGGT GGACCAGGGA GAAGGCAGGG TCGAGGGCGG GGCCGGGCCA 2581 GGAGGCCCCC TGGGAAGCCC AACCCCAGAC GGCCCAAGGA CAAGATGTCA GCCCTGGCCG 2641 CCTACTTGTA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA 2701 CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC 2761 GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCGT CGTGGCATAA CCTCCCTACT 2821 ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt