Construct: ORF TRCN0000470178
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007800.1_s317c1
- Derived from:
- ccsbBroadEn_01209
- DNA Barcode:
- AACCTTAGACTCTTCCGCTCCCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PITX1 (5307)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470178
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5307 | PITX1 | paired like homeodomain 1 | NM_002653.5 | 100% | 100% | |
| 2 | mouse | 18740 | Pitx1 | paired-like homeodomain tra... | NM_011097.2 | 91.2% | 96.5% | (many diffs) |
| 3 | mouse | 18740 | Pitx1 | paired-like homeodomain tra... | XM_006517157.3 | 91.2% | 96.5% | (many diffs) |
| 4 | mouse | 18740 | Pitx1 | paired-like homeodomain tra... | XM_006517158.3 | 91.2% | 96.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1008
- ORF length:
- 942
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgccttcaag gggggcatga gcctggagcg gctgccggag gggctccggc 121 cgccgccgcc gccaccccat gacatggggc ccgccttcca cctggcccgg cccgccgacc 181 cccgcgagcc gctcgagaac tccgccagcg agtcgtctga cacggagctg ccagagaagg 241 agcgcggcgg ggaacccaag gggcccgagg acagtggtgc gggaggcacg ggctgcggcg 301 gcgcagacga cccagccaag aagaagaagc agcggcggca acgtacgcac ttcacaagcc 361 agcagttgca agagctagag gccacgttcc agaggaaccg ctaccccgac atgagcatga 421 gggaggagat cgccgtgtgg accaacctca ccgagccgcg cgtgcgggtc tggttcaaga 481 accggcgagc caagtggcgt aagcgcgagc gtaaccagca gctggacctg tgcaagggtg 541 gctacgtgcc gcagttcagc ggcctagtgc agccctacga ggacgtgtac gccgccggct 601 actcctacaa caactgggcc gccaagagcc tggcgccagc gccgctctcc accaagagct 661 tcaccTTCTT CAACTCCATG AGCCCGCTGT CGTCGCAGTC CATGTTCTCA GCACCCAGCT 721 CCATCTCCTC CATGACCATG CCGTCCAGCA TGGGCCCAGG CGCCGTGCCT GGCATGCCCA 781 ACTCGGGCCT CAACAACATC AACAACCTCA CCGGCTCCTC GCTCAACTCG GCCATGTCGC 841 CGGGCGCTTG CCCGTACGGC ACTCCCGCCT CGCCCTACAG CGTCTACCGG GACACGTGCA 901 ACTCGAGCCT AGCCAGCCTG CGGCTCAAGT CCAAACAGCA CTCGTCGTTT GGCTACGGCG 961 GCCTGCAGGG CCCGGCCTCG GGCCTCAACG CGTGCCAGTA CAACAGCTGC CCAACTTTCT 1021 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1081 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1141 GTGGAAAGGA CGAAACCTTA GACTCTTCCG CTCCCTTACG CGTTAAGTCg acaatcaacc 1201 tctggattac aaaatttgtg aaagatt