Construct: ORF TRCN0000470182
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010362.1_s317c1
- Derived from:
- ccsbBroadEn_08399
- DNA Barcode:
- AAACACATGCGTTAAATACGACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM106B (54664)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470182
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54664 | TMEM106B | transmembrane protein 106B | NM_001134232.2 | 99.8% | 99.6% | 6A>N |
| 2 | human | 54664 | TMEM106B | transmembrane protein 106B | NM_018374.4 | 99.8% | 99.6% | 6A>N |
| 3 | human | 54664 | TMEM106B | transmembrane protein 106B | XM_005249789.1 | 99.8% | 99.6% | 6A>N |
| 4 | mouse | 71900 | Tmem106b | transmembrane protein 106B | NM_027992.3 | 88.3% | 95.2% | (many diffs) |
| 5 | mouse | 71900 | Tmem106b | transmembrane protein 106B | XM_006505169.3 | 88.3% | 95.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 888
- ORF length:
- 822
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg naagtctctt tctcatttgc ctttgcattc aagcaaagaa gatgcttatg 121 atggagtcac atctgaaaac atgaggaatg gactggttaa tagtgaagtc cataatgaag 181 atggaagaaa tggagatgtc tctcagtttc catatgtgga atttacagga agagatagtg 241 tcacctgccc tacttgtcag ggaacaggaa gaattcctag ggggcaagaa aaccaactgg 301 tggcattgat tccatatagt gatcagagat taaggccaag aagaacaaag ctgtatgtga 361 tggcttctgt gtttgtctgt ctactccttt ctggattggc tgtgtttttc cttttccctc 421 gctctatcga cgtgaaatac attggtgtaa aatcagccta tgtcagttat gatgttcaga 481 agcgtacaat ttatttaaat atcacaaaca cactaaatat aacaaacaat aactattact 541 ctgtcgaagt tgaaaacatc actgcccaag ttcaattttc aaaaacagtt attggaaagg 601 cacgcttaaa CAACATAACC ATTATTGGTC CACTTGATAT GAAACAAATT GATTACACAG 661 TACCTACCGT TATAGCAGAG GAAATGAGTT ATATGTATGA TTTCTGTACT CTGATATCCA 721 TCAAAGTGCA TAACATAGTA CTCATGATGC AAGTTACTGT GACAACAACA TACTTTGGCC 781 ACTCTGAACA GATATCCCAG GAGAGGTATC AGTATGTCGA CTGTGGAAGA AACACAACTT 841 ATCAGTTGGG GCAGTCTGAA TATTTAAATG TACTTCAGCC ACAACAGTAC CCAACTTTCT 901 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 961 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1021 GTGGAAAGGA CGAAAACACA TGCGTTAAAT ACGACTGACG CGTTAAGTCg acaatcaacc 1081 tctggattac aaaatttgtg aaagatt