Construct: ORF TRCN0000470186

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006282.1_s317c1
Derived from:
ccsbBroadEn_01307
DNA Barcode:
TACGGCGATAGCCATTACACTTCG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSMA1 (5682)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470186
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5682 PSMA1 proteasome 20S subunit alpha 1 NM_002786.3 100% 100%
2 human 5682 PSMA1 proteasome 20S subunit alpha 1 NM_148976.2 97.6% 97.3% 1_18del;20A>T
3 human 5682 PSMA1 proteasome 20S subunit alpha 1 NM_001143937.1 47.2% 44.8% (many diffs)
4 mouse 26440 Psma1 proteasome (prosome, macrop... NM_011965.2 92% 98% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
855
ORF length:
789
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt tcgaaatcag tatgacaatg atgtcactgt ttggagcccc cagggcagga
121ttcatcaaat tgaatatgca atggaagctg ttaaacaagg ttcagccaca gttggtctga
181aatcaaaaac tcatgcagtt ttggttgcat tgaaaagggc gcaatcagag cttgcagctc
241atcagaaaaa aattctccat gttgacaacc atattggtat ctcaattgcg gggcttactg
301ctgatgctag actgttatgt aattttatgc gtcaggagtg tttggattcc agatttgtat
361tcgatagacc actgcctgtg tctcgtcttg tatctctaat tggaagcaag acccagatac
421caacacaacg atatggccgg agaccatatg gtgttggtct ccttattgct ggttatgatg
481atatgggccc tcacattttc caaacctgtc catctgctaa ctattttgac tgcagagcca
541tgtccattgg agcccgttcc caatcagctc gtacttactt GGAGAGACAT ATGTCTGAAT
601TTATGGAGTG TAATTTAAAT GAACTAGTTA AACATGGTCT GCGTGCCTTA AGAGAGACGC
661TTCCTGCAGA ACAGGACCTG ACTACAAAGA ATGTTTCCAT TGGAATTGTT GGTAAAGACT
721TGGAGTTTAC AATCTATGAT GATGATGATG TGTCTCCATT CCTGGAAGGT CTTGAAGAAA
781GACCACAGAG AAAGGCACAG CCTGCTCAAC CTGCTGATGA ACCTGCAGAA AAGGCTGATG
841AACCAATGGA ACATTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC
901CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA
961TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TACGGCGATA GCCATTACAC
1021TTCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)