Construct: ORF TRCN0000470188
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012906.1_s317c1
- Derived from:
- ccsbBroadEn_13821
- DNA Barcode:
- ACTCTAACATCACAACCATTCACC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CALM1 (801)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470188
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 801 | CALM1 | calmodulin 1 | NM_001363669.1 | 99.4% | 95.5% | 110T>C;329delT |
| 2 | human | 805 | CALM2 | calmodulin 2 | NM_001305625.1 | 84.9% | 95.5% | (many diffs) |
| 3 | human | 805 | CALM2 | calmodulin 2 | NM_001305626.1 | 84.9% | 95.5% | (many diffs) |
| 4 | human | 808 | CALM3 | calmodulin 3 | NM_001329921.1 | 81.4% | 95.5% | (many diffs) |
| 5 | human | 808 | CALM3 | calmodulin 3 | NM_001329923.1 | 81.4% | 95.5% | (many diffs) |
| 6 | human | 808 | CALM3 | calmodulin 3 | NM_001329924.2 | 81.4% | 95.5% | (many diffs) |
| 7 | human | 808 | CALM3 | calmodulin 3 | NM_001329925.2 | 81.4% | 95.5% | (many diffs) |
| 8 | human | 808 | CALM3 | calmodulin 3 | NM_001329926.2 | 81.4% | 95.5% | (many diffs) |
| 9 | human | 801 | CALM1 | calmodulin 1 | NM_006888.6 | 75.3% | 72.4% | 1_108del;218T>C;437delT |
| 10 | human | 801 | CALM1 | calmodulin 1 | NM_001363670.1 | 74.8% | 72% | 1_111del;221T>C;440delT |
| 11 | human | 805 | CALM2 | calmodulin 2 | NM_001743.6 | 64.4% | 72.4% | (many diffs) |
| 12 | human | 808 | CALM3 | calmodulin 3 | NM_001329922.1 | 61.7% | 72.4% | (many diffs) |
| 13 | human | 808 | CALM3 | calmodulin 3 | NM_005184.4 | 61.7% | 72.4% | (many diffs) |
| 14 | human | 805 | CALM2 | calmodulin 2 | NM_001305624.1 | 48.7% | 54.8% | (many diffs) |
| 15 | mouse | 12314 | Calm2 | calmodulin 2 | NM_001355703.1 | 83.4% | 95.5% | (many diffs) |
| 16 | mouse | 12313 | Calm1 | calmodulin 1 | NM_009790.5 | 70.4% | 72.4% | (many diffs) |
| 17 | mouse | 12314 | Calm2 | calmodulin 2 | NM_007589.5 | 63.3% | 72.4% | (many diffs) |
| 18 | mouse | 12315 | Calm3 | calmodulin 3 | NM_007590.3 | 61.9% | 72.4% | (many diffs) |
| 19 | mouse | 12313 | Calm1 | calmodulin 1 | NM_001313934.1 | 53.2% | 54.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 405
- ORF length:
- 339
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGAG GTCACTGGGT CAGAACCCAA CAGAAGCTGA ATTGCAGGAT ATGATCAATG 121 AAGTGGATGC TGATGGTAAT GGCACCATTG ACTTCCCCGA ATTTTTGACT ATGACGGCTA 181 GAAAAATGAA AGATACAGAT AGTGAAGAAG AAATCCGTGA GGCATTCCGA GTCTTTGACA 241 AGGATGGCAA TGGTTATATC AGTGCAGCAG AACTACGTCA CGTCATGACA AACTTAGGAG 301 AAAAACTAAC AGATGAAGAA GTAGATGAAA TGATCAGAGA AGCAGATATT GATGGAGACG 361 GACAAGTCAA CTATGAAGAA TTCGTACAGA TGAGACTGCA AAATGCCCAA CTTTCTTGTA 421 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 481 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 541 AAAGGACGAA CTCTAACATC ACAACCATTC ACCACGCGTT AAGTCgacaa tcaacctctg 601 gattacaaaa tttgtgaaag att