Construct: ORF TRCN0000470211
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016401.1_s317c1
- Derived from:
- ccsbBroadEn_02442
- DNA Barcode:
- TCTGTACGATAGGTTCAACATTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ECI2 (10455)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470211
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | NM_001166010.1 | 100% | 100% | |
| 2 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | NM_006117.2 | 100% | 100% | |
| 3 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | NM_206836.2 | 92.3% | 92.3% | 1_90del |
| 4 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | XM_017010170.1 | 91.7% | 91.7% | 704_705ins90 |
| 5 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | XM_006714957.2 | 84.7% | 84.7% | 1_90del;794_795ins90 |
| 6 | human | 10455 | ECI2 | enoyl-CoA delta isomerase 2 | NR_028588.1 | 73.7% | 1_117del;527_597del;1281_1481del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1158
- ORF length:
- 1092
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tagaacagca atgagagcca gtcagaagga ctttgaaaat tcaatgaatc 121 aagtgaaact cttgaaaaag gatccaggaa acgaagtgaa gctaaaactc tacgcgctat 181 ataagcaggc cactgaagga ccttgtaaca tgcccaaacc aggtgtattt gacttgatca 241 acaaggccaa atgggacgca tggaatgccc ttggcagcct gcccaaggaa gctgccaggc 301 agaactatgt ggatttggtg tccagtttga gtccttcatt ggaatcctct agtcaggtgg 361 agcctggaac agacaggaaa tcaactgggt ttgaaactct ggtggtgacc tccgaagatg 421 gcatcacaaa gatcatgttc aaccggccca aaaagaaaaa tgccataaac actgagatgt 481 atcatgaaat tatgcgtgca cttaaagctg ccagcaagga tgactcaatc atcactgttt 541 taacaggaaa tggtgactat tacagtagtg ggaatgatct gactaacttc actgatattc 601 cccctggtgg agtagaggag aaagctaaaa ataatgccgt tttactgagg gaatttgtgg 661 gctgttttat agattttcct aagcctctga ttgcagtggt caatggtcca gctgtgggca 721 tcTCCGTCAC CCTCCTTGGG CTATTCGATG CCGTGTATGC ATCTGACAGG GCAACATTTC 781 ATACACCATT TAGTCACCTA GGCCAAAGTC CGGAAGGATG CTCCTCTTAC ACTTTTCCGA 841 AGATAATGAG CCCAGCCAAG GCAACAGAGA TGCTTATTTT TGGAAAGAAG TTAACAGCGG 901 GAGAGGCATG TGCTCAAGGA CTTGTTACTG AAGTTTTCCC TGATAGCACT TTTCAGAAAG 961 AAGTCTGGAC CAGGCTGAAG GCATTTGCAA AGCTTCCCCC AAATGCCTTG AGAATTTCAA 1021 AAGAGGTAAT CAGGAAAAGA GAGAGAGAAA AACTACACGC TGTTAATGCT GAAGAATGCA 1081 ATGTCCTTCA GGGAAGATGG CTATCAGATG AATGCACAAA TGCTGTGGTG AACTTCTTAT 1141 CCAGAAAATC AAAACTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1201 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1261 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCTGTAC GATAGGTTCA 1321 ACATTTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt