Construct: ORF TRCN0000470211

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016401.1_s317c1
Derived from:
ccsbBroadEn_02442
DNA Barcode:
TCTGTACGATAGGTTCAACATTTG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ECI2 (10455)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470211
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10455 ECI2 enoyl-CoA delta isomerase 2 NM_001166010.1 100% 100%
2 human 10455 ECI2 enoyl-CoA delta isomerase 2 NM_006117.2 100% 100%
3 human 10455 ECI2 enoyl-CoA delta isomerase 2 NM_206836.2 92.3% 92.3% 1_90del
4 human 10455 ECI2 enoyl-CoA delta isomerase 2 XM_017010170.1 91.7% 91.7% 704_705ins90
5 human 10455 ECI2 enoyl-CoA delta isomerase 2 XM_006714957.2 84.7% 84.7% 1_90del;794_795ins90
6 human 10455 ECI2 enoyl-CoA delta isomerase 2 NR_028588.1 73.7% 1_117del;527_597del;1281_1481del
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1158
ORF length:
1092
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa tagaacagca atgagagcca gtcagaagga ctttgaaaat tcaatgaatc
121aagtgaaact cttgaaaaag gatccaggaa acgaagtgaa gctaaaactc tacgcgctat
181ataagcaggc cactgaagga ccttgtaaca tgcccaaacc aggtgtattt gacttgatca
241acaaggccaa atgggacgca tggaatgccc ttggcagcct gcccaaggaa gctgccaggc
301agaactatgt ggatttggtg tccagtttga gtccttcatt ggaatcctct agtcaggtgg
361agcctggaac agacaggaaa tcaactgggt ttgaaactct ggtggtgacc tccgaagatg
421gcatcacaaa gatcatgttc aaccggccca aaaagaaaaa tgccataaac actgagatgt
481atcatgaaat tatgcgtgca cttaaagctg ccagcaagga tgactcaatc atcactgttt
541taacaggaaa tggtgactat tacagtagtg ggaatgatct gactaacttc actgatattc
601cccctggtgg agtagaggag aaagctaaaa ataatgccgt tttactgagg gaatttgtgg
661gctgttttat agattttcct aagcctctga ttgcagtggt caatggtcca gctgtgggca
721tcTCCGTCAC CCTCCTTGGG CTATTCGATG CCGTGTATGC ATCTGACAGG GCAACATTTC
781ATACACCATT TAGTCACCTA GGCCAAAGTC CGGAAGGATG CTCCTCTTAC ACTTTTCCGA
841AGATAATGAG CCCAGCCAAG GCAACAGAGA TGCTTATTTT TGGAAAGAAG TTAACAGCGG
901GAGAGGCATG TGCTCAAGGA CTTGTTACTG AAGTTTTCCC TGATAGCACT TTTCAGAAAG
961AAGTCTGGAC CAGGCTGAAG GCATTTGCAA AGCTTCCCCC AAATGCCTTG AGAATTTCAA
1021AAGAGGTAAT CAGGAAAAGA GAGAGAGAAA AACTACACGC TGTTAATGCT GAAGAATGCA
1081ATGTCCTTCA GGGAAGATGG CTATCAGATG AATGCACAAA TGCTGTGGTG AACTTCTTAT
1141CCAGAAAATC AAAACTGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1201TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1261GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCTGTAC GATAGGTTCA
1321ACATTTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)