Construct: ORF TRCN0000470219
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011151.1_s317c1
- Derived from:
- ccsbBroadEn_00103
- DNA Barcode:
- ATGGTTGTCCGTTCGGTAATTCTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RHOH (399)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470219
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 399 | RHOH | ras homolog family member H | NM_001278359.1 | 100% | 100% | |
| 2 | human | 399 | RHOH | ras homolog family member H | NM_001278360.1 | 100% | 100% | |
| 3 | human | 399 | RHOH | ras homolog family member H | NM_001278361.1 | 100% | 100% | |
| 4 | human | 399 | RHOH | ras homolog family member H | NM_001278362.1 | 100% | 100% | |
| 5 | human | 399 | RHOH | ras homolog family member H | NM_001278363.1 | 100% | 100% | |
| 6 | human | 399 | RHOH | ras homolog family member H | NM_001278364.1 | 100% | 100% | |
| 7 | human | 399 | RHOH | ras homolog family member H | NM_001278365.2 | 100% | 100% | |
| 8 | human | 399 | RHOH | ras homolog family member H | NM_001278366.2 | 100% | 100% | |
| 9 | human | 399 | RHOH | ras homolog family member H | NM_001278367.1 | 100% | 100% | |
| 10 | human | 399 | RHOH | ras homolog family member H | NM_001278368.2 | 100% | 100% | |
| 11 | human | 399 | RHOH | ras homolog family member H | NM_001278369.1 | 100% | 100% | |
| 12 | human | 399 | RHOH | ras homolog family member H | NM_004310.5 | 100% | 100% | |
| 13 | human | 399 | RHOH | ras homolog family member H | XM_011513692.1 | 100% | 100% | |
| 14 | human | 399 | RHOH | ras homolog family member H | XM_017008188.1 | 100% | 100% | |
| 15 | human | 399 | RHOH | ras homolog family member H | XM_017008189.1 | 100% | 100% | |
| 16 | human | 399 | RHOH | ras homolog family member H | XM_024454042.1 | 100% | 100% | |
| 17 | mouse | 74734 | Rhoh | ras homolog family member H | NM_001081105.1 | 90.5% | 96.8% | (many diffs) |
| 18 | mouse | 74734 | Rhoh | ras homolog family member H | XM_011240779.2 | 90.5% | 96.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 639
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gagttccatc aagtgcgtgt tggtgggcga ctctgctgtg gggaaaacct 121 ctctgttggt gcgcttcacc TCCGAGACCT TCCCGGAGGC CTACAAGCCC ACAGTGTACG 181 AGAACACAGG GGTGGACGTC TTCATGGATG GCATCCAGAT CAGCCTGGGC CTCTGGGACA 241 CAGCCGGCAA TGACGCCTTC AGAAGCATCC GGCCCCTGTC CTACCAGCAG GCAGACGTGG 301 TGCTGATGTG CTACTCTGTG GCCAACCATA ACTCATTCCT GAACTTGAAG AACAAGTGGA 361 TTGGTGAAAT TAGGAGCAAC TTGCCCTGTA CCCCTGTGCT GGTGGTGGCC ACCCAGACTG 421 ACCAGCGGGA GATGGGGCCC CACAGGGCCT CCTGCGTCAA TGCCATGGAA GGGAAGAAAC 481 TGGCCCAGGA TGTCAGAGCC AAGGGCTACC TGGAGTGCTC AGCCCTTAGC AATCGGGGAG 541 TACAGCAGGT GTTTGAGTGC GCCGTCCGAA CTGCCGTCAA CCAGGCCAGG AGACGAAACA 601 GAAGGAGGCT CTTCTCCATC AATGAGTGCA AGATCTTCTA CCCAACTTTC TTGTACAAAG 661 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 721 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 781 ACGAATGGTT GTCCGTTCGG TAATTCTGAC GCGTTAAGTC gacaatcaac ctctggatta 841 caaaatttgt gaaagatt