Construct: ORF TRCN0000470223
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003807.1_s317c1
- Derived from:
- ccsbBroadEn_15758
- DNA Barcode:
- GAACTAGAACGTTTAAATTAGATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NUDCD3 (23386)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470223
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23386 | NUDCD3 | NudC domain containing 3 | XM_017011909.1 | 87.1% | 86.8% | 0_1ins84;2T>G |
| 2 | human | 23386 | NUDCD3 | NudC domain containing 3 | XM_017011910.1 | 87.1% | 86.8% | 0_1ins84;2T>G |
| 3 | human | 23386 | NUDCD3 | NudC domain containing 3 | NM_015332.4 | 60.9% | 60.9% | 1_423del |
| 4 | human | 23386 | NUDCD3 | NudC domain containing 3 | XM_011515247.2 | 53.1% | 52% | (many diffs) |
| 5 | human | 23386 | NUDCD3 | NudC domain containing 3 | XM_017011908.1 | 28.2% | 24% | (many diffs) |
| 6 | mouse | 209586 | Nudcd3 | NudC domain containing 3 | NM_173748.4 | 53.1% | 55% | (many diffs) |
| 7 | mouse | 209586 | Nudcd3 | NudC domain containing 3 | XM_006514603.2 | 41.9% | 42.9% | (many diffs) |
| 8 | mouse | 209586 | Nudcd3 | NudC domain containing 3 | XR_001779940.1 | 34.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 726
- ORF length:
- 660
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccatggttca caggaggcag aagctccagg agcagttgct ggtgctgctg 121 aagtccctag ggaaccacca attcttccca ggattcagga gcagttccag aaaaatcccg 181 acagttacaa tggtgctgtc cgagagaact acacctggTC ACAGGACTAT ACTGACCTGG 241 AGGTCAGGGT GCCAGTACCC AAGCACGTGG TGAAGGGAAA GCAGGTCTCA GTGGCCCTTA 301 GCAGCAGCTC CATTCGTGTG GCCATGCTGG AGGAAAATGG GGAGCGCGTC CTCATGGAAG 361 GGAAGCTCAC CCACAAGATC AACACTGAGA GTTCTCTCTG GAGTCTCGAG CCCGGGAAGT 421 GCGTTTTGGT GAACCTGAGC AAGGTGGGCG AGTATTGGTG GAACGCCATC CTGGAGGGAG 481 AAGAGCCCAT CGACATTGAC AAGATCAACA AGGAGCGCTC CATGGCCACC GTGGATGAGG 541 AGGAACAGGC GGTGTTGGAC AGGCTTACCT TTGACTACCA CCAGAAGCTG CAGGGCAAGC 601 CACAGAGCCA TGAGCTGAAA GTCCATGAGA TGCTGAAGAA GGGGTGGGAT GCTGAAGGTT 661 CTCCCTTCCG AGGCCAGCGA TTCGACCCTG CCATGTTCAA CATCTCCCCG GGGGCTGTGC 721 AGTTTTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 781 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 841 CTTGGCTTTA TATATCTTGT GGAAAGGACG AGAACTAGAA CGTTTAAATT AGATAACGCG 901 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt