Construct: ORF TRCN0000470225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015676.1_s317c1
- Derived from:
- ccsbBroadEn_06366
- DNA Barcode:
- GGCGCGTCTAACTGGCTTTCCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HIP1 (3092)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470225
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3092 | HIP1 | huntingtin interacting prot... | NM_005338.7 | 99.9% | 100% | 2496A>G;2790C>T |
| 2 | human | 3092 | HIP1 | huntingtin interacting prot... | XM_011516116.2 | 99.9% | 100% | 2496A>G;2790C>T |
| 3 | human | 3092 | HIP1 | huntingtin interacting prot... | XM_005250304.2 | 96.8% | 96.5% | (many diffs) |
| 4 | human | 3092 | HIP1 | huntingtin interacting prot... | XM_017012099.1 | 96.7% | 96.3% | (many diffs) |
| 5 | human | 3092 | HIP1 | huntingtin interacting prot... | XM_005250305.2 | 96.4% | 96.1% | (many diffs) |
| 6 | human | 3092 | HIP1 | huntingtin interacting prot... | NM_001243198.3 | 95% | 95% | 2405_2406ins153;2637C>T |
| 7 | mouse | 215114 | Hip1 | huntingtin interacting prot... | NM_146001.2 | 84.2% | 87.9% | (many diffs) |
| 8 | mouse | 215114 | Hip1 | huntingtin interacting prot... | XM_011240878.2 | 84.2% | 87.9% | (many diffs) |
| 9 | mouse | 215114 | Hip1 | huntingtin interacting prot... | XM_011240879.2 | 81.9% | 84% | (many diffs) |
| 10 | mouse | 215114 | Hip1 | huntingtin interacting prot... | XM_006504415.3 | 81.1% | 84.2% | (many diffs) |
| 11 | mouse | 215114 | Hip1 | huntingtin interacting prot... | XR_868560.2 | 64% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3180
- ORF length:
- 3111
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatcggatg gccagctcca tgaagcaggt gcccaaccca ctgcccaagg 121 tgctgagccg gcgcggggtc ggcgctgggc tggaggcggc ggagcgcgag agcttcgagc 181 ggactcagac tgtcagcatc aataaggcca ttaatacgca ggaagtggct gtaaaggaaa 241 aacacgccag aacgtgcata ctgggcaccc accatgagaa aggggcacag accttctggt 301 ctgttgtcaa ccgcctgcct ctgtctagca acgcagtgct ctgctggaag ttctgccatg 361 tgttccacaa actcctccga gatggacacc cgaacgtcct gaaggactct ctgagataca 421 gaaatgaatt gagtgacatg agcaggatgt ggggccacct gagcgagggg tatggccagc 481 tgtgcagcat ctacctgaaa ctgctaagaa ccaagatgga gtaccacacc aaaaatccca 541 ggttcccagg caacctgcag atgagtgacc gccagctgga cgaggctgga gaaagtgacg 601 tgaacaactt tttccagtta acagtggaga tgtttgacta cctggagtgt gaactcaacc 661 tcttccaaac agtattcaac tccctggaca tgtcccgctc tgtgtccgtg acggcagcag 721 ggcagtgccg cctcgccccg ctgatccagg tcatcttgga ctgcagccac ctttatgact 781 acactgtcaa gcttctcttc aaactccact cctgcctccc agctgacacc ctgcaaggcc 841 accgggaccg cttcatggag cagtttacaa agttgaaaga tctgttctac cgctccagca 901 acctgcagta cttcaagcgg ctcattcaga tcccccagct gcctgagaac ccacccaact 961 tcctgcgagc ctcagccctg tcagaacata tcagccctgt ggtggtgatc cctgcagagg 1021 cctcatcccc cgacagcgag ccagtcctag agaaggatga cctcatggac atggatgcct 1081 ctcagcagaa tttatttgac aacaagtttg atgacatctt tggcagttca ttcagcagtg 1141 atcccttcaa tttcaacagt caaaatggtg tgaacaagga tgagaaggac cacttaattg 1201 agcgactata cagagagatc agtggattga aggcacagct agaaaacatg aagactgaga 1261 gccagcgggt tgtgctgcag ctgaagggcc acgtcagcga gctggaagca gatctggccg 1321 agcagcagca cctgcggcag caggcggccg acgactgtga attcctgcgg gcagaactgg 1381 acgagctcag gaggcagcgg gaggacaccg agaaggctca gcggagcctg tctgagatag 1441 aaaggaaagc tcaagccaat gaacagcgat atagcaagct aaaggagaag tacagcgagc 1501 tggttcagaa ccacgctgac ctgctgcgga agaatgcaga ggtgaccaaa caggtgtcca 1561 tggccagaca agcccaggta gatttggaac gagagaaaaa agagctggag gattcgttgg 1621 agcgcatcag tgaccagggc cagcggaaga ctcaagaaca gctggaagtt ctagagagct 1681 tgaagcagga acttgccaca agccaacggg agcttcaggt tctgcaaggc agcctggaaa 1741 cttctgccca gtcagaagca aactgggcag ccgagttcgc cgagctagag aaggagcggg 1801 acagcctggt gagtggcgca gctcataggg aggaggaatt atctgctctt cggaaagaac 1861 tgcaggacac tcagctcaaa ctggccagca cagaggaatc tatgtgccag cttgccaaag 1921 accaacgaaa aatgcttctg gtggggtcca ggaaggctgc ggagcaggtg atacaagacg 1981 ccctgaacca gcttgaagaa cctcctctca tcagctgcgc tgggtctgca gatcacctcc 2041 tctccacggt cacatccatt tccagctgca tcgagcaact ggagaaaagc tggagccagt 2101 atctggcctg cccagaagac atcagtggac ttctccattc cataaccctg ctggcccact 2161 tgaccagcga cgccattgct catggtgcca ccacctgcct cagagcccca cctgagcctg 2221 ccgactcact gaccgaggcc tgtaagcagt atggcaggga aaccctcgcc tacctggcct 2281 ccctggagga agagggaagc cttgagaatg ccgacagcac agccatgagg aactgcctga 2341 gcaagatcaa ggccatcggc gaggagctcc tgcccagggg actggacatc aagcaggagg 2401 agctggggga cctggtggac aaggagatgg cggccacttc agctgctatt gaaactgcca 2461 cggccagaat agaggagatg ctcagcaaat cccgagcagg agacacagga gtcaaattgg 2521 aggtgaatga aaggatcctt ggttgctgta ccagcctcat gcaggctatt caggtgctca 2581 tcgtggcctc taaggacctc cagagagaga ttgtggagag cggcaggggt acagcatccc 2641 ctaaagagtt ttatgccaag aactctcgat ggacagaagg acttatctca gccTCCAAGG 2701 CTGTGGGCTG GGGAGCCACT GTCATGGTGG ATGCAGCTGA TCTGGTGGTA CAAGGCAGAG 2761 GGAAATTTGA GGAGCTAATG GTGTGTTCTC ATGAAATTGC TGCTAGCACA GCCCAGCTTG 2821 TGGCTGCATC CAAGGTGAAA GCTGATAAGG ACAGCCCTAA CCTAGCCCAG CTGCAGCAGG 2881 CCTCTCGGGG AGTGAACCAG GCCACTGCCG GCGTTGTGGC CTCAACCATT TCCGGCAAAT 2941 CACAGATCGA AGAGACAGAC AACATGGACT TCTCAAGCAT GACGCTGACA CAGATCAAAC 3001 GCCAAGAGAT GGATTCTCAG GTTAGGGTGC TAGAGCTAGA AAATGAATTG CAGAAGGAGC 3061 GTCAAAAACT GGGAGAGCTT CGGAAAAAGC ACTACGAGCT TGCTGGTGTT GCTGAGGGCT 3121 GGGAAGAAGG AACAGAGGCA TCTCCACCTA CACTGCAAGA AGTGGTAACC GAAAAAGAAT 3181 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3241 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3301 TTTATATATC TTGTGGAAAG GACGAGGCGC GTCTAACTGG CTTTCCGACA CGCGTTAAGT 3361 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt