Construct: ORF TRCN0000470233
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012413.1_s317c1
- Derived from:
- ccsbBroadEn_10735
- DNA Barcode:
- TGCAGCCACTTTTTTAGGCACCTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERCC8 (1161)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470233
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1161 | ERCC8 | ERCC excision repair 8, CSA... | NM_001007234.3 | 99.8% | 100% | 435T>C |
| 2 | human | 1161 | ERCC8 | ERCC excision repair 8, CSA... | NM_000082.3 | 50.5% | 46.3% | (many diffs) |
| 3 | human | 1161 | ERCC8 | ERCC excision repair 8, CSA... | NM_001007233.2 | 35.8% | 31.9% | (many diffs) |
| 4 | mouse | 71991 | Ercc8 | excision repaiross-compleme... | XM_017315608.1 | 79.2% | 78% | (many diffs) |
| 5 | mouse | 71991 | Ercc8 | excision repaiross-compleme... | XM_011244697.2 | 72.8% | 71.5% | (many diffs) |
| 6 | mouse | 71991 | Ercc8 | excision repaiross-compleme... | XR_001780818.1 | 50% | (many diffs) | |
| 7 | mouse | 71991 | Ercc8 | excision repaiross-compleme... | NM_028042.3 | 44.5% | 42.5% | (many diffs) |
| 8 | mouse | 71991 | Ercc8 | excision repaiross-compleme... | XM_006517763.3 | 20.2% | 17.4% | (many diffs) |
| 9 | mouse | 71991 | Ercc8 | excision repaiross-compleme... | XR_382744.3 | 10.1% | (many diffs) | |
| 10 | mouse | 71991 | Ercc8 | excision repaiross-compleme... | XR_382743.3 | 10.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 681
- ORF length:
- 615
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct ggggtttttg tccgcacgcc aaacgggttt ggaggaccct cttcgccttc 121 ggagagcaga gtcaacacgg agagttttgg gactggaatt aaataaagac agagatgttg 181 aaagaatcca cggcggtgga attaacaccc ttgacattga acctgttGAA GGGAGATACA 241 TGTTATCAGG TGGTTCAGAT GGTGTGATTG TACTTTATGA CCTTGAGAAC TCCAGCAGAC 301 AATCTTATTA CACATGTAAA GCAGTGTGTT CCATTGGCAG AGATCATCCT GATGTTCACA 361 GATACAGTGT GGAGACTGTA CAGTGGTATC CTCATGACAC TGGCATGTTC ACATCAAGCT 421 CATTTGATAA AACTCTGAAA GTATGGGATA CAAATACATT ACAAACTGCA GATGTATTTA 481 ATTTTGAGGA AACAGTTTAC AGTCATCATA TGTCTCCAGT CTCCACCAAG CACTGTTTGG 541 TAGCAGTTGG TACTAGAGGA CCCAAAGTAC AACTTTGTGA CTTGAAGTCT GGATCCTGTT 601 CTCACATTCT ACAGGGTATT TTTATTTTAT TTCAAACGGC AACTACTTTG AGTAAACGAT 661 TCAATAAAAA GAAACGTTAC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 721 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 781 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATGCA GCCACTTTTT 841 TAGGCACCTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt