Construct: ORF ccsbBroadEn_10735

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012413.1_s300c1, BRDN0000387444
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ERCC8 (1161)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1161 ERCC8 ERCC excision repair 8, CSA... NM_001007234.3 99.8% 100% 435T>C
2 human 1161 ERCC8 ERCC excision repair 8, CSA... NM_000082.3 50.5% 46.3% (many diffs)
3 human 1161 ERCC8 ERCC excision repair 8, CSA... NM_001007233.2 35.8% 31.9% (many diffs)
4 mouse 71991 Ercc8 excision repaiross-compleme... XM_017315608.1 79.2% 78% (many diffs)
5 mouse 71991 Ercc8 excision repaiross-compleme... XM_011244697.2 72.8% 71.5% (many diffs)
6 mouse 71991 Ercc8 excision repaiross-compleme... XR_001780818.1 50% (many diffs)
7 mouse 71991 Ercc8 excision repaiross-compleme... NM_028042.3 44.5% 42.5% (many diffs)
8 mouse 71991 Ercc8 excision repaiross-compleme... XM_006517763.3 20.2% 17.4% (many diffs)
9 mouse 71991 Ercc8 excision repaiross-compleme... XR_382744.3 10.1% (many diffs)
10 mouse 71991 Ercc8 excision repaiross-compleme... XR_382743.3 10.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
681
ORF length:
615
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG
61TTGGCATGCT GGGGTTTTTG TCCGCACGCC AAACGGGTTT GGAGGACCCT CTTCGCCTTC
121GGAGAGCAGA GTCAACACGG AGAGTTTTGG GACTGGAATT AAATAAAGAC AGAGATGTTG
181AAAGAATCCA CGGCGGTGGA ATTAACACCC TTGACATTGA ACCTGTTGAA GGGAGATACA
241TGTTATCAGG TGGTTCAGAT GGTGTGATTG TACTTTATGA CCTTGAGAAC TCCAGCAGAC
301AATCTTATTA CACATGTAAA GCAGTGTGTT CCATTGGCAG AGATCATCCT GATGTTCACA
361GATACAGTGT GGAGACTGTA CAGTGGTATC CTCATGACAC TGGCATGTTC ACATCAAGCT
421CATTTGATAA AACTCTGAAA GTATGGGATA CAAATACATT ACAAACTGCA GATGTATTTA
481ATTTTGAGGA AACAGTTTAC AGTCATCATA TGTCTCCAGT CTCCACCAAG CACTGTTTGG
541TAGCAGTTGG TACTAGAGGA CCCAAAGTAC AACTTTGTGA CTTGAAGTCT GGATCCTGTT
601CTCACATTCT ACAGGGTATT TTTATTTTAT TTCAAACGGC AACTACTTTG AGTAAACGAT
661TCAATAAAAA GAAACGTTAC TACCCAACTT TCTTGTACAA AGTtggcatt ataagaaagc
721attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)