Construct: ORF TRCN0000470236
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018114.1_s317c1
- Derived from:
- ccsbBroadEn_04516
- DNA Barcode:
- GAACGGCCGGACAGTTGTGGCTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCDC32 (90416)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470236
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90416 | CCDC32 | coiled-coil domain containi... | NM_001080792.3 | 100% | 100% | |
| 2 | human | 90416 | CCDC32 | coiled-coil domain containi... | NM_052849.4 | 100% | 100% | |
| 3 | human | 90416 | CCDC32 | coiled-coil domain containi... | NM_001080791.3 | 95.3% | 95.3% | 1_27del |
| 4 | human | 90416 | CCDC32 | coiled-coil domain containi... | NM_001289132.1 | 72.2% | 71.8% | 402_402delGins154 |
| 5 | mouse | 269336 | Ccdc32 | coiled-coil domain containi... | NM_199310.2 | 85.7% | 83.2% | (many diffs) |
| 6 | mouse | 269336 | Ccdc32 | coiled-coil domain containi... | XM_011239574.1 | 85.7% | 83.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 621
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa aatgtttgag agcgctgact ctacagccac aagatctggc caggatctct 121 gggctgaaat ttgttcctgt ctgccaaatc ctgaacaaga agatggtgcc aacaatgcat 181 tcTCAGACTC CTTTGTGGAT TCTTGCCCTG AAGGTGAAGG CCAGAGGGAG GTGGCTGACT 241 TTGCTGTCCA GCCAGCTGTA AAGCCTTGGG CTCCCTTGCA GGATTCAGAA GTGTATTTAG 301 CATCTCTAGA GAAGAAGCTA AGAAGAATCA AAGGTTTAAA TCAGGAAGTG ACTTCCAAGG 361 ACATGCTTCG AACTCTGGCC CAAGCCAAGA AGGAATGCTG GGATCGGTTC CTCCAGGAGA 421 AGTTAGCTTC AGAGTTCTTT GTGGATGGAC TTGATTCTGA TGAGAGCACC TTGGAACATT 481 TCAAGAGGTG GCTCCAGCCA GATAAAGTAG CCGTCAGCAC AGAGGAGGTC CAGTATCTGA 541 TTCCTCCAGA GTCACAGGTT GAGAAGCCAG TGGCCGAGGA CGAGCCAGCA GCCGGGGACA 601 AGCCAGCAGC AGCAGAACAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 661 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 721 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGAAC GGCCGGACAG 781 TTGTGGCTTT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt