Construct: ORF TRCN0000470242
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009136.1_s317c1
- Derived from:
- ccsbBroadEn_02247
- DNA Barcode:
- TTCATGAGAAGCGCGTGTGCCCGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOMM20 (9804)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470242
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9804 | TOMM20 | translocase of outer mitoch... | NM_014765.3 | 100% | 100% | |
| 2 | human | 493754 | GS1-124K5.11 | RAB guanine nucleotide exch... | NR_111974.1 | 23.7% | (many diffs) | |
| 3 | human | 493754 | GS1-124K5.11 | RAB guanine nucleotide exch... | NR_111975.1 | 22.4% | (many diffs) | |
| 4 | human | 493754 | GS1-124K5.11 | RAB guanine nucleotide exch... | NR_111979.1 | 21.5% | (many diffs) | |
| 5 | human | 493754 | GS1-124K5.11 | RAB guanine nucleotide exch... | NR_111976.1 | 21.5% | (many diffs) | |
| 6 | human | 493754 | GS1-124K5.11 | RAB guanine nucleotide exch... | NR_111978.1 | 20.4% | (many diffs) | |
| 7 | human | 493754 | GS1-124K5.11 | RAB guanine nucleotide exch... | NR_111972.1 | 20.4% | (many diffs) | |
| 8 | human | 493754 | GS1-124K5.11 | RAB guanine nucleotide exch... | NR_111977.1 | 19.7% | (many diffs) | |
| 9 | mouse | 67952 | Tomm20 | translocase of outer mitoch... | NM_024214.2 | 92.1% | 99.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttGGCATGGT GGGTCGGAAC AGCGCCATCG CCGCCGGTGT ATGCGGGGCC CTTTTCATTG 121 GGTACTGCAT CTACTTCGAC CGCAAAAGAC GAAGTGACCC CAACTTCAAG AACAGGCTTC 181 GAGAACGAAG AAAGAAACAG AAGCTTGCCA AGGAGAGAGC TGGGCTTTCC AAGTTACCTG 241 ACCTTAAAGA TGCTGAAGCT GTTCAGAAGT TCTTCCTTGA AGAAATACAG CTTGGTGAAG 301 AGTTACTAGC TCAAGGTGAA TATGAGAAGG GCGTAGACCA TCTGACAAAT GCAATTGCTG 361 TGTGTGGACA GCCACAGCAG TTACTGCAGG TCTTACAGCA AACTCTTCCA CCACCAGTGT 421 TCCAGATGCT TCTGACTAAG CTCCCAACAA TTAGTCAGAG AATTGTAAGT GCTCAGAGCT 481 TGGCTGAAGA TGATGTGGAA TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATTCA TGAGAAGCGC 661 GTGTGCCCGG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt