Construct: ORF TRCN0000470253
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017703.1_s317c1
- Derived from:
- ccsbBroadEn_00714
- DNA Barcode:
- AAGGTCACGTGCCGCATGCTCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- H1-2 (3006)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470253
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3006 | H1-2 | H1.2 linker histone, cluste... | NM_005319.3 | 100% | 100% | |
| 2 | human | 3008 | H1-4 | H1.4 linker histone, cluste... | NM_005321.2 | 77.4% | 84.5% | (many diffs) |
| 3 | mouse | 50708 | Hist1h1c | histone cluster 1, H1c | NM_015786.3 | 81.6% | 88.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cgagactgct cctgccgctc ccgctgccgc gcctcctgcg gagaaggccc 121 ctgtaaagaa gaaggcggcc aaaaaggctg ggggtacgcc tcgtaaggcg tctggtcccc 181 cggtgtcaga gctcatcacc aaggctgtgg ccgcctctaa agagcgtagc ggagtttctc 241 tggctgctct gaaaaaagcg ttggctgccg ccggctatga tgtggagaaa aacaacagcc 301 gtatcaaact tggtctcaag agccTGGTGA GCAAGGGCAC TCTGGTGCAA ACGAAAGGCA 361 CCGGTGCTTC TGGCTCCTTT AAACTCAACA AGAAGGCAGC CTCCGGGGAA GCCAAGCCCA 421 AGGTTAAAAA GGCGGGCGGA ACCAAACCTA AGAAGCCAGT TGGGGCAGCC AAGAAGCCCA 481 AGAAGGCGGC TGGCGGCGCA ACTCCGAAGA AGAGCGCTAA GAAAACACCG AAGAAAGCGA 541 AGAAGCCGGC CGCGGCCACT GTAACCAAGA AAGTGGCTAA GAGCCCAAAG AAGGCCAAGG 601 TTGCGAAGCC CAAGAAAGCT GCCAAAAGTG CTGCTAAGGC TGTGAAGCCC AAGGCCGCTA 661 AGCCCAAGGT TGTCAAGCCT AAGAAGGCGG CGCCCAAGAA GAAATACCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA AAGGTCACGT GCCGCATGCT CGACACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt