Construct: ORF TRCN0000470275
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000215.1_s317c1
- Derived from:
- ccsbBroadEn_05335
- DNA Barcode:
- GTCCGTTTAAGAACGTTTCGGCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SELENOH (280636)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470275
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 280636 | SELENOH | selenoprotein H | NM_001321335.1 | 100% | 100% | |
| 2 | human | 280636 | SELENOH | selenoprotein H | NM_170746.4 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 195
- ORF length:
- 129
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tCCCCGCGGG AGGAAGCGTA AGGCTGAGGC CGCGGTGGTC GCCGTAGCCG 121 AGAAGCGAGA GAAGCTGGCG AACGGCGGGG AGGGAATGGA GGAGGCGACC GTTGTTATCG 181 AGCATTGCAC TAGCTGACGC GTCTATGGGC GCAACGCCGC GGCCCTGAGC CAGGCGCTGC 241 GCCTGGAGGC CCCAGAGCTT CCAGTAAAGG TGAACCCGAC GAAGCCCCGG AGGGGCAGCT 301 TCGAGGTGAC GCTGCTGCGC CCGGACGGCA GCAGTGCGGA GCTCTGGACT GGGATTAAGA 361 AGGGGCCCCC ACGCAAACTC AAATTCCCTG AGCCTCAAGA GGTGGTGGAA GAGTTGAAGA 421 AGTACCTGTC GTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 481 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 541 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTC CGTTTAAGAA CGTTTCGGCA 601 CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t