Construct: ORF TRCN0000470291
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008241.1_s317c1
- Derived from:
- ccsbBroadEn_00794
- DNA Barcode:
- TGGGTAATAACTGTGAGCGGGGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSF1 (3297)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470291
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3297 | HSF1 | heat shock transcription fa... | NM_005526.4 | 100% | 100% | |
| 2 | human | 3297 | HSF1 | heat shock transcription fa... | XM_005272315.3 | 94.9% | 94.7% | 1143_1226del |
| 3 | human | 3297 | HSF1 | heat shock transcription fa... | XM_017013377.2 | 92.4% | 86.4% | 1381_1382insCAGGGAAG;1467_1468ins112 |
| 4 | human | 3297 | HSF1 | heat shock transcription fa... | XM_005272316.3 | 87.7% | 82% | 1143_1226del;1465_1466insCAGGGAAG;1551_1552ins112 |
| 5 | human | 3297 | HSF1 | heat shock transcription fa... | XR_001745527.2 | 72.9% | 1_155del;1013_1053del;1784_2176del | |
| 6 | human | 3297 | HSF1 | heat shock transcription fa... | XR_246618.4 | 65.9% | 1_155del;1013_1014ins179;1564_1956del | |
| 7 | human | 3297 | HSF1 | heat shock transcription fa... | XR_001745526.2 | 65.5% | (many diffs) | |
| 8 | human | 3297 | HSF1 | heat shock transcription fa... | XM_024447144.1 | 60.1% | 60.1% | 0_1ins633 |
| 9 | human | 3297 | HSF1 | heat shock transcription fa... | XM_005272317.2 | 58.6% | 53.5% | 858_859ins179;930_931ins478 |
| 10 | human | 3297 | HSF1 | heat shock transcription fa... | XM_011517004.2 | 57% | 56.9% | 0_1ins633;510_593del |
| 11 | human | 3297 | HSF1 | heat shock transcription fa... | XM_011517006.2 | 43.8% | 43.4% | 0_1ins849;2_3insAGACGA;288_371del |
| 12 | mouse | 15499 | Hsf1 | heat shock factor 1 | NM_001331154.1 | 87% | 89.6% | (many diffs) |
| 13 | mouse | 15499 | Hsf1 | heat shock factor 1 | NM_008296.3 | 83% | 85.6% | (many diffs) |
| 14 | mouse | 15499 | Hsf1 | heat shock factor 1 | NM_001331152.1 | 82.6% | 84.9% | (many diffs) |
| 15 | mouse | 15499 | Hsf1 | heat shock factor 1 | NM_001331153.1 | 78.9% | 81.1% | (many diffs) |
| 16 | mouse | 15499 | Hsf1 | heat shock factor 1 | NM_001331214.1 | 71% | 72.2% | (many diffs) |
| 17 | mouse | 15499 | Hsf1 | heat shock factor 1 | NR_138588.1 | 52.2% | (many diffs) | |
| 18 | mouse | 15499 | Hsf1 | heat shock factor 1 | NR_138589.1 | 45.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1653
- ORF length:
- 1587
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tctgcccgtg ggccccggcg cggcggggcc cagcaacgtc ccggccttcc 121 tgaccaagct gtggaccctc gtgagcgacc cggacaccga cgcgctcatc tgctggagcc 181 cgagcgggaa cagcttccac gtgttcgacc agggccagtt tgccaaggag gtgctgccca 241 agtacttcaa gcacaacaac atggccagct tcgtgcggca gctcaacatg tatggcttcc 301 ggaaagtggt ccacatcgag cagggcggcc tggtcaagcc agagagagac gacacggagt 361 tccagcaccc atgcttcctg cgtggccagg agcagctcct tgagaacatc aagaggaaag 421 tgaccagtgt gtccaccctg aagagtgaag acataaagat ccgccaggac agcgtcacca 481 agctgctgac ggacgtgcag ctgatgaagg ggaagcagga gtgcatggac tccaagctcc 541 tggccatgaa gcatgagaat gaggctctgt ggcgggaggt ggccagcctt cggcagaagc 601 atgcccagca acagaaagtc gtcaacaagc tcattcagtt cctgatctca ctggtgcagt 661 caaaccggat cctgggggtg aagagaaaga tccccctgat gctgaacgac agtggctcag 721 cacattccat gcccaagtat agccggcagt tctccctgga gcacgtccac ggctcgggcc 781 cctactcggc cccctcccca gcctacagca gctccagcct ctacgcccct gatgctgtgg 841 ccagctctgg acccatcatc tccgacatca ccgagctggc tcctgccagc cccatggcct 901 cccccggcgg gagcatagac gagaggcccc tatccagcag ccccctggtg cgtgtcaagg 961 aggagccccc cagcccgcct cagagccccc gggtagagga ggcgagtccc gggcgcccat 1021 cttccgtgga caccctcttg tccccgaccg ccctcattga ctccatcctg cgggagagtg 1081 aacctgcccc cgcctccgtc acagccctca cggacgccag gggccacacg gacaccgagg 1141 gccggcctcc ctcccccccg cccacctcca cccctgaaaa gtgccTCAGC GTAGCCTGCC 1201 TGGACAAGAA TGAGCTCAGT GACCACTTGG ATGCTATGGA CTCCAACCTG GATAACCTGC 1261 AGACCATGCT GAGCAGCCAC GGCTTCAGCG TGGACACCAG TGCCCTGCTG GACCTGTTCA 1321 GCCCCTCGGT GACCGTGCCC GACATGAGCC TGCCTGACCT TGACAGCAGC CTGGCCAGTA 1381 TCCAAGAGCT CCTGTCTCCC CAGGAGCCCC CCAGGCCTCC CGAGGCAGAG AACAGCAGCC 1441 CGGATTCAGG GAAGCAGCTG GTGCACTACA CAGCGCAGCC GCTGTTCCTG CTGGACCCCG 1501 GCTCCGTGGA CACCGGGAGC AACGACCTGC CGGTGCTGTT TGAGCTGGGA GAGGGCTCCT 1561 ACTTCTCCGA AGGGGACGGC TTCGCCGAGG ACCCCACCAT CTCCCTGCTG ACAGGCTCGG 1621 AGCCTCCCAA AGCCAAGGAC CCCACTGTCT CCTACCCAAC TTTCTTGTAC AAAGTGGTTG 1681 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1741 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 1801 GGTAATAACT GTGAGCGGGG TTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1861 ttgtgaaaga tt