Construct: ORF TRCN0000470295

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018250.1_s317c1
Derived from:
ccsbBroadEn_06798
DNA Barcode:
CCACAGGCCTCTAGATGTACTACA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PSMB1 (5689)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470295
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5689 PSMB1 proteasome 20S subunit beta 1 NM_002793.4 99.8% 99.5% 31C>G
2 mouse 19170 Psmb1 proteasome (prosome, macrop... NM_011185.3 83.8% 92.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
789
ORF length:
723
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtt gtcctctaca gccatgtatt cggctgctgg cagagacttg gggatggaac
121cgcacagagc cgcgggccct ttgcagctgc gattttcgcc ctacgttttc aacggaggta
181ctatactggc aattgctgga gaagattttg caattgttgc ttctgatact cgattgagtg
241aagggttttc aattcatacg cgggatagcc ccaaatgtta caaattaaca gacaaaacag
301tcattGGATG CAGCGGTTTT CATGGAGACT GTCTTACGCT GACAAAGATT ATTGAAGCAA
361GACTAAAGAT GTATAAGCAT TCCAATAATA AGGCCATGAC TACGGGGGCA ATTGCTGCAA
421TGCTGTCTAC AATCCTGTAT TCAAGGCGCT TCTTTCCATA CTATGTTTAC AACATCATCG
481GTGGACTTGA TGAAGAAGGA AAGGGGGCTG TATACAGCTT TGATCCAGTA GGGTCTTACC
541AGAGAGACTC CTTCAAGGCT GGAGGCTCAG CAAGTGCCAT GCTACAGCCC CTGCTTGACA
601ACCAGGTTGG TTTTAAGAAC ATGCAGAATG TGGAGCATGT TCCGCTGTCC TTGGACAGAG
661CCATGCGGCT GGTGAAAGAT GTCTTCATTT CTGCGGCTGA GAGAGATGTG TACACTGGGG
721ACGCACTCCG GATCTGCATA GTGACCAAAG AGGGCATCAG GGAGGAAACT GTTTCCTTAA
781GGAAGGACTG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
841CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
901TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACCACAG GCCTCTAGAT GTACTACAAC
961GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)