Construct: ORF TRCN0000470313
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017422.1_s317c1
- Derived from:
- ccsbBroadEn_11818
- DNA Barcode:
- ACTCCGAGAGACGGAGCACACTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RIBC2 (26150)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470313
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26150 | RIBC2 | RIB43A domain with coiled-c... | XM_005261524.4 | 100% | 100% | |
| 2 | human | 26150 | RIBC2 | RIB43A domain with coiled-c... | NM_015653.5 | 80.8% | 80.8% | 1_219del |
| 3 | human | 26150 | RIBC2 | RIB43A domain with coiled-c... | XM_011530126.2 | 68.6% | 63% | (many diffs) |
| 4 | human | 26150 | RIBC2 | RIB43A domain with coiled-c... | XM_017028766.1 | 30.3% | 29.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 993
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcaaaatgac aaaatcatgt gcatattgga aaaccggaaa aagagggata 121 ggaaaaatct ctgtagggct atcaatgact tccaacagag ctttcagaag ccagaaactc 181 gccgtgaatt tgatctgtcc gaccccctag cccttaagaa agatcttcca gcccggcagt 241 cagataatga tgttcggaat acgatatcag gaatgcagaa attcatggga gaggatttaa 301 acttccatga gaggaagaaa ttccaagagg aacaaaacag agaatggtct ttgcagcagc 361 aaagggaatg gaagaacgcc cgtgctgaac aaaaatgcgc agaggccctc tacacagaga 421 caaggctgca gtttgacgag acagccaagc acctccagaa gctggaaagc accaccagaa 481 aggcagtttg tgcatctgtg aaagacttca acaagagcca ggccatcgag tcagtggaaa 541 ggaaaaagca agagaaaaag caagaacaag aggacaactt ggccgagatc accaacctcc 601 tgcgtgggga cctgctcTCC GAGAACCCGC AGCAGGCAGC CAGCTCCTTC GGGCCCCACC 661 GCGTGGTCCC TGACCGCTGG AAGGGCATGA CCCAGGAGCA GCTGGAGCAG ATCCGCCTAG 721 TCCAGAAGCA GCAAATCCAG GAGAAGCTGA GGCTCCAGGA AGAAAAGCGC CAGCGAGACC 781 TGGACTGGGA CCGGCGGAGG ATTCAGGGGG CTCGCGCCAC CCTGCTGTTT GAGCGGCAGC 841 AGTGGCGGCG GCAGCGCGAC CTGCGCAGAG CTCTGGACAG CAGCAACCTC AGCCTGGCCA 901 AGGAGCAGCA TTTGCAGAAA AAATATATGA ATGAAGTCTA TACAAATCAA CCCACGGGAG 961 ACTATTTCAC ACAATTTAAT ACAGGAAGTC GATACCCAAC TTTCTTGTAC AAAGTGGTTG 1021 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1081 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAAC 1141 TCCGAGAGAC GGAGCACACT ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 1201 ttgtgaaaga tt