Construct: ORF TRCN0000470336
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017626.1_s317c1
- Derived from:
- ccsbBroadEn_11620
- DNA Barcode:
- GCCGTGAGGGCACGAAACATGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GPN1 (11321)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470336
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11321 | GPN1 | GPN-loop GTPase 1 | NM_007266.4 | 100% | 100% | |
| 2 | human | 11321 | GPN1 | GPN-loop GTPase 1 | NM_001145047.1 | 93.1% | 90.7% | (many diffs) |
| 3 | human | 11321 | GPN1 | GPN-loop GTPase 1 | NM_001145048.1 | 78.8% | 78.8% | 0_1ins237 |
| 4 | human | 11321 | GPN1 | GPN-loop GTPase 1 | NM_001145049.1 | 74.5% | 71.6% | 0_1ins178;26_27ins107 |
| 5 | human | 11321 | GPN1 | GPN-loop GTPase 1 | NR_026735.1 | 54.8% | 1_63del;267_268ins107;1079_1742del | |
| 6 | mouse | 74254 | Gpn1 | GPN-loop GTPase 1 | NM_133756.4 | 86.5% | 90.3% | (many diffs) |
| 7 | mouse | 74254 | Gpn1 | GPN-loop GTPase 1 | XM_006504161.3 | 78.3% | 82.8% | (many diffs) |
| 8 | mouse | 74254 | Gpn1 | GPN-loop GTPase 1 | XM_006504162.2 | 68.7% | 72.4% | (many diffs) |
| 9 | mouse | 74254 | Gpn1 | GPN-loop GTPase 1 | XM_011240774.2 | 68.7% | 72.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1188
- ORF length:
- 1122
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgtccgca gctgccgctg agctccaggc ttctgggggt ccgcggcacc 121 cagtgtgtct gttggtgttg ggaatggcgg gatccgggaa aaccactttt gtacagaggc 181 tcacaggaca cctgcatgcc caaggcactc caccgtatgt gatcaacctg gatccagcag 241 tacatgaagt tccctttcct gccaatattg atattcgtga tactgtaaag tataaagaag 301 taatgaaaca atatggactt ggacccaatg gcggcatagt gacctcactc aatctctttg 361 ctaccagatt tgatcaggtg atgaaattta ttgagaaggc ccagaacatg tccaaatatg 421 tgttgattga cacacctgga cagattgagg tattcacctg gtcagcttct gggacaatta 481 tcactgaagc ccttgcatcc tcatttccaa cagttgtcat ctatgtaatg gacacatcga 541 gaagtaccaa cccagtgacc ttcatgtcca acatgctcta tgcctgcagc atcttataca 601 aaaccaagct gcctttcatt gtggtcatga ataaaactga catcattgac cacagctttg 661 cagtggaatg gatgcaggat tttgaggctt tccaagatgc cttgaatcaa gagactacat 721 acgtcagtaa CCTGACTCGT TCAATGAGCC TGGTGTTAGA TGAGTTTTAC AGCTCACTCA 781 GGGTGGTGGG TGTCTCTGCT GTTCTGGGTA CTGGATTAGA TGAACTCTTT GTGCAAGTTA 841 CCAGTGCTGC CGAAGAATAT GAAAGGGAGT ATCGTCCTGA ATATGAACGT CTGAAAAAAT 901 CACTGGCCAA CGCAGAGAGC CAACAGCAGA GAGAACAACT GGAACGCCTT CGAAAAGATA 961 TGGGTTCTGT AGCCTTGGAT GCAGGGACTG CCAAAGACAG CTTATCTCCT GTGCTGCACC 1021 CTTCTGATTT GATCCTGACT CGAGGAACCT TGGATGAAGA GGATGAGGAA GCAGACAGCG 1081 ATACTGATGA CATTGACCAC AGAGTTACAG AGGAAAGCCA TGAAGAGCCA GCATTCCAGA 1141 ATTTTATGCA AGAATCGATG GCACAATACT GGAAGAGAAA CAATAAATAC CCAACTTTCT 1201 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1261 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1321 GTGGAAAGGA CGAGCCGTGA GGGCACGAAA CATGGATACG CGTTAAGTCg acaatcaacc 1381 tctggattac aaaatttgtg aaagatt