Construct: ORF TRCN0000470338
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010357.1_s317c1
- Derived from:
- ccsbBroadEn_01757
- DNA Barcode:
- GTGCGCGTGGTAGTGGACAGCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UQCRC2 (7385)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470338
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7385 | UQCRC2 | ubiquinol-cytochrome c redu... | NM_003366.4 | 100% | 100% | |
| 2 | mouse | 67003 | Uqcrc2 | ubiquinol cytochrome c redu... | NM_025899.2 | 86% | 85.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1425
- ORF length:
- 1359
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gctactaacc agagccggct ctttctcgag attttattcc ctcaaagttg 121 cccccaaagt taaagccaca gctgcgcctg caggagcacc gccacaacct caggaccttg 181 agtttaccaa gttaccaaat ggcttggtga ttgcttcttt ggaaaactat tctcctgtat 241 caagaattgg tttgttcatt aaagcaggca gtagatatga ggacttcagc aatttaggaa 301 ccacccattt gctgcgtctt acatccagtc tgacgacaaa aggagcttca tctttcaaga 361 taacccgtgg aattgaagca gttggtggca aattaagtgt gaccgcaaca agggaaaaca 421 tggcttatac tgtggaatgc ctgcggggtg atgttgatat tctaatggag ttcctgctca 481 atgtcaccac agcaccagaa tttcgtcgtt gggaagtagc tgaccttcag cctcagctaa 541 agattgacaa agctgtggcc tttcagaatc cgcagactca tgtcattgaa aatttgcatg 601 cagcagctta ccggaatgcc ttggctaatc ccttgtattg tcctgactat aggattggaa 661 aagtgacatc agaggagtta cattacttcg ttcagaacca tttcacaagt gcaagaatgg 721 ctttgattgg acttggtgtg agtcatcctg ttctaaagca agttgctgaa cagtttctca 781 acatgagggg tgggcttggt ttatctggtg caaaggccaa ctaccgtgga ggtgaaatcc 841 gagaacagaa tggagacagt cttgtccatg ctgcttttgt agcagaaagt gctgtcgcgg 901 gaagtgcaga ggcaaatgca tttagtgttc ttcagcatgt cctcggtgct gggCCACATG 961 TCAAGAGGGG CAGCAACACC ACCAGCCATC TGCACCAGGC TGTTGCCAAG GCAACTCAGC 1021 AGCCATTTGA TGTTTCTGCA TTTAATGCCA GTTACTCAGA TTCTGGACTC TTTGGGATTT 1081 ATACTATCTC CCAGGCCACA GCTGCTGGAG ATGTTATCAA GGCTGCCTAT AATCAAGTAA 1141 AAACAATAGC TCAAGGAAAC CTTTCCAACA CAGATGTCCA AGCTGCCAAG AACAAGCTGA 1201 AAGCTGGATA CCTAATGTCA GTGGAGTCTT CTGAGTGTTT CCTGGAAGAA GTCGGGTCCC 1261 AGGCTCTAGT TGCTGGTTCT TACATGCCAC CATCCACAGT CCTTCAGCAG ATTGATTCAG 1321 TGGCTAATGC TGATATCATA AATGCGGCAA AGAAGTTTGT TTCTGGCCAG AAGTCAATGG 1381 CAGCAAGTGG AAATTTGGGA CATACACCTT TTGTTGATGA GTTGTACCCA ACTTTCTTGT 1441 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1501 AGTAATGAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG 1561 TGCGCGTGGT AGTGGACAGC GACACGCGTT AAGTCgacaa tcaacctctg gattacaaaa 1621 tttgtgaaag att