Construct: ORF TRCN0000470345

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016343.1_s317c1
Derived from:
ccsbBroadEn_00757
DNA Barcode:
TTTATAAACCATCACTACACGCGA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HMGA1 (3159)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470345
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319077.2 100% 100%
2 human 3159 HMGA1 high mobility group AT-hook 1 NM_002131.4 100% 100%
3 human 3159 HMGA1 high mobility group AT-hook 1 NM_145902.3 100% 100%
4 human 3159 HMGA1 high mobility group AT-hook 1 NM_145903.3 100% 100%
5 human 3159 HMGA1 high mobility group AT-hook 1 NM_145905.2 100% 100%
6 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319080.2 90.2% 89.6% 103_133delinsA
7 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319078.2 89.7% 89.7% 102_134del
8 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319079.2 89.7% 89.7% 102_134del
9 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319081.2 89.7% 89.7% 102_134del
10 human 3159 HMGA1 high mobility group AT-hook 1 NM_001319082.2 89.7% 89.7% 102_134del
11 human 3159 HMGA1 high mobility group AT-hook 1 NM_145899.3 89.7% 89.7% 102_134del
12 human 3159 HMGA1 high mobility group AT-hook 1 NM_145901.2 89.7% 89.7% 102_134del
13 mouse 111241 Hmga1-rs1 high mobility group AT-hook... NM_001166477.1 92% 96.8% (many diffs)
14 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166537.1 92% 96.8% (many diffs)
15 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166539.1 92% 96.8% (many diffs)
16 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166540.1 92% 96.8% (many diffs)
17 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166541.1 92% 96.8% (many diffs)
18 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166542.1 92% 96.8% (many diffs)
19 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001025427.3 82.5% 86.9% (many diffs)
20 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001039356.2 82.5% 86.9% (many diffs)
21 mouse 111241 Hmga1-rs1 high mobility group AT-hook... NM_001166476.1 82.5% 86.9% (many diffs)
22 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166535.1 82.5% 86.9% (many diffs)
23 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166536.1 82.5% 86.9% (many diffs)
24 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_016660.3 82.5% 86.9% (many diffs)
25 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166543.1 81.6% 85.9% (many diffs)
26 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166544.1 81.6% 85.9% (many diffs)
27 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166545.1 79.5% 83.7% (many diffs)
28 mouse 15361 Hmga1 high mobility group AT-hook 1 NM_001166546.1 73.1% 57.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
183
ORF end:
471
ORF length:
288
Sequence:
1GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA TTCTCCTTGG AATTTGCCCT
61TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA GTGGTTCAAA GTTTTTTTCT
121TCCATTTCAG GTGTCGTGAG GCTAGCATCG ATTGATCAAC AAGTTTGTAC AAAAAAGTTG
181GCATGAGTGA GTCGAGCTCG AAGTCCAGCC AGCCCTTGGC CTCCAAGCAG GAAAAGGACG
241GCACTGAGAA GCGGGGCCGG GGCAGGCCGC GCAAGCAGCC TCCGAAGGAG CCCAGCGAAG
301TGCCAACACC TAAGAGACCT CGGGGCCGAC CAAAGGGAAG CAAAAACAAG GGTGCTGCCA
361AGACCCGGAA AACCACCACA ACTCCAGGAA GGAAACCAAG GGGCAGACCC AAAAAACTGG
421AGAAGGAGGA AGAGGAGGGC ATCTCGCAGG AGTCCTCGGA GGAGGAGCAG TGCCCAACTT
481TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT
541CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT
601CTTGTGGAAA GGACGATTTA TAAACCATCA CTACACGCGA ACGCGTTAAG TCgacaatca
661acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)

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