Construct: ORF TRCN0000470347
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001273.1_s317c1
- Derived from:
- ccsbBroadEn_02134
- DNA Barcode:
- GATTTCGACCCTGGACTCATTCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NREP (9315)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470347
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142476.1 | 100% | 100% | |
| 2 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142477.1 | 100% | 100% | |
| 3 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142478.2 | 100% | 100% | |
| 4 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142479.1 | 100% | 100% | |
| 5 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142480.1 | 100% | 100% | |
| 6 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142481.1 | 100% | 100% | |
| 7 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142482.1 | 100% | 100% | |
| 8 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142483.1 | 100% | 100% | |
| 9 | human | 9315 | NREP | neuronal regeneration relat... | NM_004772.4 | 100% | 100% | |
| 10 | human | 9315 | NREP | neuronal regeneration relat... | XM_006714733.2 | 77.7% | 77% | 1_55delinsA;58_60delTCT |
| 11 | human | 9315 | NREP | neuronal regeneration relat... | XM_006714732.1 | 72.7% | 72% | 1_73delinsA;76_78delTCT |
| 12 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142474.2 | 66.6% | 65.6% | 1_95del;98_104delGTTTCCA |
| 13 | human | 9315 | NREP | neuronal regeneration relat... | NM_001142475.2 | 60.4% | 59.8% | 1_132delinsA;135delT |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 239
- ORF end:
- 443
- ORF length:
- 204
- Sequence:
-
1 TCGTCTTTAG GTTGGGGGGA GGGGTTTTAT GCGATGGAGT TTCCCCACAC TGAGTGGGTG 61 GAGACTGAAG TTAGGCCAGC TTGGCACTTG ATGTAATTCT CCTTGGAATT TGCCCTTTTT 121 GAGTTTGGAT CTTGGTTCAT TCTCAAGCCT CAGACAGTGG TTCAAAGTTT TTTTCTTCCA 181 TTTCAGGTGT CGTGAGGCTA GCATCGATTG ATCAACAAGT TTGTACAAAA AAGTTGGCAT 241 GGTTTATTAC CCAGAACTCT TTGTCTGGGT CAGTCAAGAA CCATTTCCAA ACAAGGACAT 301 GGAGGGAAGG CTTCCTAAGG GAAGACTTCC TGTCCCAAAG GAAGTGAACC GCAAGAAGAA 361 CGATGAGACA AACGCTGCCT CCCTGACTCC ACTGGGCAGC AGTGAACTCC GCTCCCCAAG 421 AATCAGTTAC CTCCACTTTT TTTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA 481 GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT 541 TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGAGA TTTCGACCCT 601 GGACTCATTC AAACGCGTTA AGTCgacaat caacctctgg attacaaaat ttgtgaaaga 661 tt