Construct: ORF TRCN0000470352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015701.1_s317c1
- Derived from:
- ccsbBroadEn_07076
- DNA Barcode:
- TCTGACGCTTAAGACCTGATTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TSPAN7 (7102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7102 | TSPAN7 | tetraspanin 7 | NM_004615.3 | 99.5% | 98.7% | 115T>C;157G>A;379G>A |
| 2 | mouse | 21912 | Tspan7 | tetraspanin 7 | NM_019634.2 | 93% | 97.1% | (many diffs) |
| 3 | mouse | 21912 | Tspan7 | tetraspanin 7 | XM_006527583.1 | 82.7% | 85.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 813
- ORF length:
- 747
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc atcgaggaga atggagacca aacctgtgat aacctgtctc aaaaccctcc 121 tcatcatcta ctccttcgtc ttctggatca ctggggtgat cctgctggct gttggagtcc 181 ggggcaaact tactctgggc acctatatct cccttattgc caagaactcc acaaatgctc 241 cctatgtgct catcggaact ggcaccacta ttgttgtctt tggcctgttt ggatgctttg 301 ctacatgtcg tggtagccca tggatgctga aactgtatgc catgtttctg tccctggtgt 361 tcctggctga gctcgtagct ggCATTTCAG GGTTTGTGTT TCGTCATGAG ATCAAGGACA 421 CCTTCCTGAG GACTTACACG GACACTATGC AGACTTACAA TGGCAATGAT GAGAGGAGCC 481 GGGCAGTGGA CCATGTGCAG CGCAGCCTGA GCTGCTGTGG TGTGCAGAAC TACACCAACT 541 GGAGCACCAG CCCCTACTTC CTGGAGCATG GCATCCCCCC CAGCTGCTGC ATGAACGAAA 601 CTGATTGTAA TCCCCAGGAT CTACACAATC TGACTGTGGC CGCCACCAAA GTTAACCAGA 661 AGGGTTGTTA TGATCTGGTA ACTAGTTTCA TGGAGACTAA CATGGGAATC ATCGCTGGAG 721 TGGCGTTTGG AATCGCATTC TCCCAGTTAA TTGGCATGCT GCTGGCCTGC TGTCTGTCCC 781 GGTTCATCAC GGCCAATCAG TATGAGATGG TGTACCCAAC TTTCTTGTAC AAAGTGGTTG 841 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 901 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 961 TGACGCTTAA GACCTGATTC CTACGCGTTA AGTCgacaat caacctctgg attacaaaat 1021 ttgtgaaaga tt