Construct: ORF TRCN0000470353
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015540.1_s317c1
- Derived from:
- ccsbBroadEn_11246
- DNA Barcode:
- CAGCGGGGTAACCCCTATTCACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB7A (7879)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470353
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7879 | RAB7A | RAB7A, member RAS oncogene ... | NM_004637.6 | 31% | 12.4% | 1_310del;621_622ins382 |
| 2 | mouse | 19349 | Rab7 | RAB7, member RAS oncogene f... | NM_001293652.1 | 28.5% | 12.4% | (many diffs) |
| 3 | mouse | 19349 | Rab7 | RAB7, member RAS oncogene f... | NM_001293653.1 | 28.5% | 12.4% | (many diffs) |
| 4 | mouse | 19349 | Rab7 | RAB7, member RAS oncogene f... | NM_001293654.1 | 28.5% | 12.4% | (many diffs) |
| 5 | mouse | 19349 | Rab7 | RAB7, member RAS oncogene f... | NM_001293655.1 | 28.5% | 12.4% | (many diffs) |
| 6 | mouse | 19349 | Rab7 | RAB7, member RAS oncogene f... | NM_009005.3 | 28.5% | 12.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 759
- ORF length:
- 693
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag tttctcatcc aggccagtcc ccgagatcct gaaaacttcc catttgttgt 121 gttgggaaac aagattgacc tcgaaaacag acaagtggcc acaaagcggg cacaggcctg 181 gtgctacagc aaaaacaaca ttccctactt tgagaccagt gccaaggagg ccatcaacgt 241 ggagcaggcg ttccagacga ttgcacggaa tgcacttaag caggaaacgg aggtggagct 301 gtacaacgaa tttcctgaac ctatcaaact ggacaagaat gaccgggcca aggcctcggc 361 agaaagcTGC AGTTGCTGAG GGGGCAGTGA GAGTTGAGCA CAGAGTCCTT CACAAACCAA 421 GAACACACGT AGGCCTTCAA CACAATTCCC CTCTCCTCTT CCAAACAAAA CATACATTGA 481 TCTCTCACAT CCAGCTGCCA AAAGAAAACC CCATCAAACA CAGTTACACC CCACATATCT 541 CTCACACACA CACACACACG CACACACACA CACACAGATC TGACGTAATC AAACTCCAGC 601 CCTTGCCCGT GATGGCTCCT TGGGGTCTGC CTGCCCACCC ACATGAGCCC GCGAGTATGG 661 CAGCAGGACA AGCCAGCGGT GGAAGTCATT CTGATATGGA GTTGGCATTG GAAGCTTATT 721 CTTTTTGTTC ACTGGAGAGA GAGAGAACTG TTTACAGTTA CCCAACTTTC TTGTACAAAG 781 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 841 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 901 ACGACAGCGG GGTAACCCCT ATTCACATAC GCGTTAAGTC gacaatcaac ctctggatta 961 caaaatttgt gaaagatt