Transcript: Human NM_004637.6

Homo sapiens RAB7A, member RAS oncogene family (RAB7A), mRNA.

Source:
NCBI, updated 2019-09-15
Taxon:
Homo sapiens (human)
Gene:
RAB7A (7879)
Length:
2185
CDS:
186..809

Additional Resources:

NCBI RefSeq record:
NM_004637.6
NBCI Gene record:
RAB7A (7879)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004637.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000218462 ACGAATTTCCTGAACCTATCA pLKO_005 736 CDS 100% 4.950 6.930 N RAB7A n/a
2 TRCN0000007998 GAAACAAGATTGACCTCGAAA pLKO.1 556 CDS 100% 4.950 6.930 N RAB7A n/a
3 TRCN0000229643 ATGGATAAATTGCCGTTATTT pLKO_005 1751 3UTR 100% 15.000 12.000 N RAB7A n/a
4 TRCN0000379950 ACCAGTATGTGAATAAGAAAT pLKO_005 262 CDS 100% 13.200 9.240 N RAB7A n/a
5 TRCN0000381534 ACGTAGGCCTTCAACACAATT pLKO_005 857 3UTR 100% 13.200 9.240 N RAB7A n/a
6 TRCN0000229642 ACTGCTGCGTTCTGGTATTTG pLKO_005 430 CDS 100% 13.200 9.240 N RAB7A n/a
7 TRCN0000229641 GGCTAGTCACAATGCAGATAT pLKO_005 349 CDS 100% 13.200 9.240 N RAB7A n/a
8 TRCN0000380432 ATAGCTGGAGAGATGAGTTTC pLKO_005 484 CDS 100% 10.800 7.560 N RAB7A n/a
9 TRCN0000218448 ATCAGTACAAAGCCACAATAG pLKO_005 289 CDS 100% 10.800 7.560 N RAB7A n/a
10 TRCN0000380577 GGTTATCATCCTGGGAGATTC pLKO_005 215 CDS 100% 10.800 7.560 N RAB7A n/a
11 TRCN0000007997 CAACGAATTTCCTGAACCTAT pLKO.1 734 CDS 100% 4.950 3.465 N RAB7A n/a
12 TRCN0000007996 GCCACAATAGGAGCTGACTTT pLKO.1 300 CDS 100% 4.950 3.465 N RAB7A n/a
13 TRCN0000100880 GCGGCAGTATTCTGTACAGTA pLKO.1 1590 3UTR 100% 4.950 3.465 N Rab7 n/a
14 TRCN0000007994 GCAATGGATAAATTGCCGTTA pLKO.1 1748 3UTR 100% 0.405 0.284 N RAB7A n/a
15 TRCN0000007995 CCCATTTGTTGTGTTGGGAAA pLKO.1 539 CDS 100% 4.050 2.430 N RAB7A n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004637.6, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_11246 pDONR223 100% 31% 12.4% None 1_310del;621_622ins382 n/a
2 ccsbBroad304_11246 pLX_304 0% 31% 12.4% V5 1_310del;621_622ins382 n/a
3 TRCN0000470353 CAGCGGGGTAACCCCTATTCACAT pLX_317 56.3% 31% 12.4% V5 1_310del;621_622ins382 n/a
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