Construct: ORF TRCN0000470360
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018224.1_s317c1
- Derived from:
- ccsbBroadEn_13637
- DNA Barcode:
- ACATAACCGTGCATCCTCATCGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC26A5 (375611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470360
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NM_206884.3 | 91.6% | 91.4% | 1311_1406del;1515_1548delinsG |
| 2 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NM_001321787.2 | 72.3% | 72.2% | 1419_1959delinsG |
| 3 | human | 375611 | SLC26A5 | solute carrier family 26 me... | XR_002956437.1 | 71.4% | (many diffs) | |
| 4 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NM_206885.3 | 70.4% | 67.5% | (many diffs) |
| 5 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NM_206883.3 | 69% | 68.9% | 1311_1406del;1515_2055delinsG |
| 6 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NM_001167962.1 | 66.3% | 66.2% | 1419_2136delinsG |
| 7 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NM_198999.3 | 63.5% | 63.4% | 1311_1406del;1515_2232delinsG |
| 8 | human | 375611 | SLC26A5 | solute carrier family 26 me... | XM_011516170.3 | 63.5% | 63.4% | 1311_1406del;1515_2232delinsG |
| 9 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NR_135802.2 | 60% | 1_292del;1712_2364del | |
| 10 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NR_120441.1 | 57.8% | 1_182del;1602_2451del | |
| 11 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NR_120443.1 | 57.2% | 1_182del;1412_1413ins78;1524_2265del | |
| 12 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NR_135801.1 | 48.8% | 1_262del;1232_1233ins148;1533_2453delinsG | |
| 13 | human | 375611 | SLC26A5 | solute carrier family 26 me... | NR_120442.1 | 47.8% | (many diffs) | |
| 14 | human | 375611 | SLC26A5 | solute carrier family 26 me... | XR_001744726.1 | 42.3% | 1_1052del;2363_2458del;2568_3352del | |
| 15 | human | 375611 | SLC26A5 | solute carrier family 26 me... | XR_001744727.2 | 17.1% | 1_262del;1682_8281del | |
| 16 | human | 375611 | SLC26A5 | solute carrier family 26 me... | XR_001744725.2 | 16.9% | 1_262del;1573_1668del;1778_8377del | |
| 17 | mouse | 80979 | Slc26a5 | solute carrier family 26, m... | NM_030727.5 | 56.4% | 61.9% | (many diffs) |
| 18 | mouse | 80979 | Slc26a5 | solute carrier family 26, m... | NM_001289787.1 | 52.1% | 57.2% | (many diffs) |
| 19 | mouse | 80979 | Slc26a5 | solute carrier family 26, m... | XM_006535818.1 | 49.2% | 54.4% | (many diffs) |
| 20 | mouse | 80979 | Slc26a5 | solute carrier family 26, m... | NM_001289788.1 | 24.5% | 27.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1488
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatcatgct gaagaaaatg aaatccttgc agcaacccag aggtactatg 121 tggaaaggcc tatctttagt catccggtcc tccaggaaag actacacaca aaggacaagg 181 ttcctgattc cattgcggat aagctgaaac aggcattcac atgtactcct aaaaaaataa 241 gaaatatcat ttatatgttc ctacccataa ctaaatggct gccagcatac aaattcaagg 301 aatatgtgtt gggtgacttg gtctcaggca taagcacagg ggtgcttcag cttcctcaag 361 gcttagcctt tgcaatgctg gcagctgtgc ctccaatatt tggcctgtac tcttcatttt 421 accctgttat catgtattgt tttcttggaa cctccagaca catatccata ggtccttttg 481 ctgttattag cctgatgatt ggtggtgtag ctgttcgatt agtaccagat gatatagtca 541 ttccaggagg agtaaatgca accaatggca cagaggccag agatgccttg agagtgaaag 601 tcgccatgtc tgtgacctta ctttcaggaa tcattcagtt ttgcctaggt gtctgtaggt 661 ttggatttgt ggccatatat ctcacagagc ctctggtccg tgggtttacc accgcagcag 721 ctgtgcatgt cttcacctcc atgttaaaat atctgtttgg agttaaaaca aagcggtaca 781 gtggaatctt ttccgtggtg tatagtacag ttgctgtgtt gcagaatgtt aaaaacctca 841 acgtgtgttc cctaggcgtc gggctgatgg tttttggttt gctgttgggt ggcaaggagt 901 ttaatgagag atttaaagag aaattgccgg cgcctattcc tttagagttc tttgcggtcg 961 taatgggaac tggcatttca gctgggttta acttgaaaga atcatacaat gtggatgtcg 1021 ttggaacact tcctctaggg ctgctacctc cagccaatcc ggacaccagc ctcttccacc 1081 ttgtgtacGT AGATGCCATT GCCATAGCCA TCGTTGGATT TTCAGTGACC ATCTCCATGG 1141 CCAAGACCTT AGCAAATAAA CATGGCTACC AGGTTGACGG CAATCAGGAG CTCATTGCCC 1201 TGGGACTGTG CAATTCCATT GGCTCACTCT TCCAGACCTT TTCAATTTCA TGCTCCTTGT 1261 CTCGAAGCCT TGTTCAGGAG GGAACCGGTG GGAAGACACA GCTTGCAGGT TGTTTGGCCT 1321 CATTAATGAT TCTGCTGGTC ATATTAGCAA CTGGATTCCT CTTTGAATCA TTGCCCCAGA 1381 CCATCTGGCT TACCACTTTT GTGTCCTCCT TGTTCCTGGG ATTGGACTAT GGTTTGATCA 1441 CTGCTGTGAT CATTGCTCTG CTGACTGTGA TTTACAGAAC ACAGAGGTTG CCAACTTTCT 1501 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1561 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1621 GTGGAAAGGA CGAACATAAC CGTGCATCCT CATCGCAACG CGTTAAGTCg acaatcaacc 1681 tctggattac aaaatttgtg aaagatt