Construct: ORF TRCN0000470365
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016183.1_s317c1
- Derived from:
- ccsbBroadEn_03510
- DNA Barcode:
- GCTAATTGACTTTGCAATCGACCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FKBP14 (55033)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470365
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55033 | FKBP14 | FKBP prolyl isomerase 14 | NM_017946.4 | 100% | 100% | |
| 2 | human | 55033 | FKBP14 | FKBP prolyl isomerase 14 | NR_046478.2 | 12.4% | 1_194del;543_634del;920_5070del | |
| 3 | human | 55033 | FKBP14 | FKBP prolyl isomerase 14 | NR_046479.2 | 9.6% | 1_194del;391_392ins152;676_4826del | |
| 4 | mouse | 231997 | Fkbp14 | FK506 binding protein 14 | NM_153573.1 | 87.3% | 92.8% | (many diffs) |
| 5 | mouse | 231997 | Fkbp14 | FK506 binding protein 14 | XR_377439.2 | 16.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 699
- ORF length:
- 633
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gcttttcttg tggaacgcgg tcttgactct gttcgtcact tctttgattg 121 gggctttgat ccctgaacca gaagtgaaaa ttgaagttct ccagaagcca ttcatctgcc 181 atcgcaagac caaaggaggg gatttgatgt tggtcCACTA TGAAGGCTAC TTAGAAAAGG 241 ACGGCTCCTT ATTTCACTCC ACTCACAAAC ATAACAATGG TCAGCCCATT TGGTTTACCC 301 TGGGCATCCT GGAGGCTCTC AAAGGTTGGG ACCAGGGCTT GAAAGGAATG TGTGTAGGAG 361 AGAAGAGAAA GCTCATCATT CCTCCTGCTC TGGGCTATGG AAAAGAAGGA AAAGGTAAAA 421 TTCCCCCAGA AAGTACACTG ATATTTAATA TTGATCTCCT GGAGATTCGA AATGGACCAA 481 GATCCCATGA ATCATTCCAA GAAATGGATC TTAATGATGA CTGGAAACTC TCTAAAGATG 541 AGGTTAAAGC ATATTTAAAG AAGGAGTTTG AAAAACATGG TGCGGTGGTG AATGAAAGTC 601 ATCATGATGC TTTGGTGGAG GATATTTTTG ATAAAGAAGA TGAAGACAAA GATGGGTTTA 661 TATCTGCCAG AGAATTTACA TATAAACACG ATGAGTTATA CCCAACTTTC TTGTACAAAG 721 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 781 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 841 ACGAGCTAAT TGACTTTGCA ATCGACCTAC GCGTTAAGTC gacaatcaac ctctggatta 901 caaaatttgt gaaagatt