Construct: ORF TRCN0000470371
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006893.1_s317c1
- Derived from:
- ccsbBroadEn_01967
- DNA Barcode:
- TCAACTTGTCATCGGTTGGGCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLPP1 (8611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470371
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8611 | PLPP1 | phospholipid phosphatase 1 | NM_003711.4 | 100% | 100% | |
| 2 | human | 8611 | PLPP1 | phospholipid phosphatase 1 | NM_176895.2 | 90.5% | 88.8% | (many diffs) |
| 3 | human | 8611 | PLPP1 | phospholipid phosphatase 1 | XM_006714724.3 | 77.8% | 75.3% | 0_1ins37;21_22ins152 |
| 4 | human | 8611 | PLPP1 | phospholipid phosphatase 1 | NR_103485.1 | 46.3% | 1_449del;508_662del;1457_1840del | |
| 5 | mouse | 19012 | Plpp1 | phospholipid phosphatase 1 | NM_008903.2 | 83.6% | 83% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 918
- ORF length:
- 852
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tgacaagacg cggctgccgt acgtggccct cgatgtgctc tgcgtgttgc 121 tggctggatt gccttttgca attcttactt caaggcatac ccccttccaa cgaggagtat 181 tctgtaatga tgagtccatc aagtaccctt acaaagaaga caccatacct tatgcgttat 241 taggtggaat aatcattcca ttcagtatta tcgttattat tcttggagaa accctgtctg 301 tttactgtaa ccttttgcac tcaaattcct ttatcaggaa taactacata gccactattt 361 acaaagccat tggaaccttt ttatttggtg cagctgctag tcagtccctg actgacattg 421 ccaagtattc aataggcaga ctgcggcctc acttcttGGA TGTTTGTGAT CCAGATTGGT 481 CAAAAATCAA CTGCAGCGAT GGTTACATTG AATACTACAT ATGTCGAGGG AATGCAGAAA 541 GAGTTAAGGA AGGCAGGTTG TCCTTCTATT CAGGCCACTC TTCGTTTTCC ATGTACTGCA 601 TGCTGTTTGT GGCACTTTAT CTTCAAGCCA GGATGAAGGG AGACTGGGCA AGACTCTTAC 661 GCCCCACACT GCAATTTGGT CTTGTTGCCG TATCCATTTA TGTGGGCCTT TCTCGAGTTT 721 CTGATTATAA ACACCACTGG AGCGATGTGT TGACTGGACT CATTCAGGGA GCTCTGGTTG 781 CAATATTAGT TGCTGTATAT GTATCGGATT TCTTCAAAGA AAGAACTTCT TTTAAAGAAA 841 GAAAAGAGGA GGACTCTCAT ACAACTCTGC ATGAAACACC AACAACTGGG AATCACTATC 901 CGAGCAATCA CCAGCCTTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 961 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1021 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATCAACTT GTCATCGGTT 1081 GGGCCTCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt