Construct: ORF TRCN0000470383
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016375.1_s317c1
- Derived from:
- ccsbBroadEn_00497
- DNA Barcode:
- GCAAAGGTCACAGATGCCTCTAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ELF5 (2001)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470383
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2001 | ELF5 | E74 like ETS transcription ... | NM_001422.4 | 100% | 100% | |
| 2 | human | 2001 | ELF5 | E74 like ETS transcription ... | NM_198381.2 | 96.2% | 96.2% | 1_30del |
| 3 | human | 2001 | ELF5 | E74 like ETS transcription ... | XM_017017308.1 | 93.3% | 92.9% | 355_356ins51 |
| 4 | human | 2001 | ELF5 | E74 like ETS transcription ... | XM_017017309.1 | 90.9% | 90.9% | 406_407ins69 |
| 5 | human | 2001 | ELF5 | E74 like ETS transcription ... | NM_001243081.1 | 66.7% | 66.7% | 1_30del;149_150ins234 |
| 6 | human | 2001 | ELF5 | E74 like ETS transcription ... | NM_001243080.2 | 62.7% | 62.3% | 121_122ins285 |
| 7 | mouse | 13711 | Elf5 | E74-like factor 5 | NM_001145813.1 | 88.1% | 94.5% | (many diffs) |
| 8 | mouse | 13711 | Elf5 | E74-like factor 5 | NM_010125.3 | 88.1% | 94.5% | (many diffs) |
| 9 | mouse | 13711 | Elf5 | E74-like factor 5 | XM_006498705.1 | 88.1% | 94.5% | (many diffs) |
| 10 | mouse | 13711 | Elf5 | E74-like factor 5 | XM_006498704.1 | 78.5% | 84.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 831
- ORF length:
- 765
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ggactcggtg acacacagca ccttcctgcc taatgcatcc ttctgcgatc 121 ccctgatgtc gtggactgat ctgttcagca atgaagagta ctaccctgcc tttgagcatc 181 agacagcctg tgactcatac tggacatcag tccaccctga atactggact aagcgccatg 241 tgtgggagtg gctccagttc tgctgcgacc agtacaagtt ggacaccaat tgcatctcct 301 tctgcaactt caacatcagt ggcctgcagc tgtgcagcat gacacaggag gagttcgtcg 361 aggcagctgg ccTCTGCGGC GAGTACCTGT ACTTCATCCT CCAGAACATC CGCACACAAG 421 GTTACTCCTT TTTTAATGAC GCTGAAGAAA GCAAGGCCAC CATCAAAGAC TATGCTGATT 481 CCAACTGCTT GAAAACAAGT GGCATCAAAA GTCAAGACTG TCACAGTCAT AGTAGAACAA 541 GCCTCCAAAG TTCTCATCTA TGGGAATTTG TACGAGACCT GCTTCTATCT CCTGAAGAAA 601 ACTGTGGCAT TCTGGAATGG GAAGATAGGG AACAAGGAAT TTTTCGGGTG GTTAAATCGG 661 AAGCCCTGGC AAAGATGTGG GGACAAAGGA AGAAAAATGA CAGAATGACA TATGAAAAGT 721 TGAGCAGAGC CCTGAGATAC TACTATAAAA CAGGAATTTT GGAGCGGGTT GACCGAAGGT 781 TAGTGTACAA ATTTGGAAAA AATGCACACG GGTGGCAGGA AGACAAGCTA TGCCCAACTT 841 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 901 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 961 CTTGTGGAAA GGACGAGCAA AGGTCACAGA TGCCTCTAAT ACGCGTTAAG TCgacaatca 1021 acctctggat tacaaaattt gtgaaagatt