Construct: ORF TRCN0000470423
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016084.1_s317c1
- Derived from:
- ccsbBroadEn_12134
- DNA Barcode:
- TTTAATTGGTCCCGACCCTGACGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCUB (55013)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470423
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55013 | MCUB | mitochondrial calcium unipo... | XM_006714246.3 | 80.6% | 80.7% | 1_177del;411T>C |
| 2 | human | 55013 | MCUB | mitochondrial calcium unipo... | NM_017918.5 | 73.7% | 73.8% | 1_264del;498T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 810
- ORF length:
- 744
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt gtcaacagtt ggttcattcc ttcaggacct acaaaatgaa gataagggta 121 tcaaaactgc agccatcttc acagcagatg gcaacatgat ttcagcttct accttgatgg 181 atattttgct aatgaatgat tttaaacttg tcattaataa aatagcatat gatgtgcagt 241 gtccaaagag agaaaaacca agtaatgagc acactgctga gatggaacac atgaaatcct 301 tggttcacag actatttaca atcttgcatt tagaagagtc tcagaaaaag agagagcacc 361 atttactgga gaaaattgac cacctgaagg aacagctgca gccCCTTGAA CAGGTGAAAG 421 CTGGAATAGA AGCTCATTCG GAAGCCAAAA CCAGTGGACT CCTGTGGGCT GGATTGGCAC 481 TGCTGTCCAT TCAGGGTGGG GCACTGGCCT GGCTCACGTG GTGGGTGTAC TCCTGGGATA 541 TCATGGAGCC AGTTACATAC TTCATCACAT TTGCAAATTC TATGGTCTTT TTTGCATACT 601 TTATAGTCAC TCGACAGGAT TATACTTACT CAGCTGTTAA GAGTAGGCAA TTTCTTCAGT 661 TCTTCCACAA GAAATCAAAG CAACAGCACT TTGATGTGCA GCAATACAAC AAGTTAAAAG 721 AAGACCTTGC TAAGGCTAAA GAATCCCTGA AACAGGCGCG TCATTCTCTC TGTTTGCAAA 781 TGCAAGTAGA AGAACTCAAT GAAAAGAATT ACCCAACTTT CTTGTACAAA GTGGTTGATA 841 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 901 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATTTAA 961 TTGGTCCCGA CCCTGACGGA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 1021 tgaaagatt