Construct: ORF TRCN0000470424

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004702.1_s317c1
Derived from:
ccsbBroadEn_06358
DNA Barcode:
ATTGACTAATAATCTATTTACCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HDGF (3068)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470424
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3068 HDGF heparin binding growth factor NM_004494.3 99.7% 100% 270G>A;648G>A
2 human 3068 HDGF heparin binding growth factor NM_001126051.1 92.1% 87.7% (many diffs)
3 human 3068 HDGF heparin binding growth factor NM_001126050.2 88.2% 81.5% (many diffs)
4 human 3068 HDGF heparin binding growth factor NM_001319187.1 86.5% 86.6% 0_1ins96;174G>A
5 human 3068 HDGF heparin binding growth factor NM_001319188.1 86.5% 86.6% 0_1ins96;174G>A
6 human 3068 HDGF heparin binding growth factor XM_011509453.2 86.3% 86.6% 0_1ins96;174G>A;552G>A
7 human 3068 HDGF heparin binding growth factor XM_011509454.2 86.3% 86.6% 0_1ins96;174G>A;552G>A
8 human 3068 HDGF heparin binding growth factor XM_011509455.2 86.3% 86.6% 0_1ins96;174G>A;552G>A
9 human 3068 HDGF heparin binding growth factor NM_001319186.2 85.8% 79.4% (many diffs)
10 human 3068 HDGF heparin binding growth factor XR_001737133.1 31.9% (many diffs)
11 human 3068 HDGF heparin binding growth factor NR_135008.2 30.4% (many diffs)
12 mouse 15191 Hdgf hepatoma-derived growth factor NM_008231.4 88% 88.3% (many diffs)
13 mouse 15191 Hdgf hepatoma-derived growth factor NM_001331052.1 75.9% 75% (many diffs)
14 mouse 15191 Hdgf hepatoma-derived growth factor XM_006501029.3 75.9% 75% (many diffs)
15 mouse 15191 Hdgf hepatoma-derived growth factor NM_001331051.1 61.8% 46.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
786
ORF length:
720
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtc gcgatccaac cggcagaagg agtacaaatg cggggacctg gtgttcgcca
121agatgaaggg ctacccacac tggccggccc ggattgacga gatgcctgag gctgccgtga
181aatcaacagc caacaaatac caagtctttt ttttcgggac ccacgagacg gcattcctgg
241gccccaaaga cctcttccct tacgaggaat ccaaggagaa gtttggcaag cccaacaaga
301ggaaagggtt cagcgagggg ctgtgggaga tcgaaaaCAA CCCTACTGTC AAGGCTTCCG
361GCTATCAGTC CTCCCAGAAA AAGAGCTGTG TGGAAGAGCC TGAACCAGAG CCCGAAGCTG
421CAGAGGGTGA CGGTGATAAG AAGGGGAATG CAGAGGGCAG CAGCGACGAG GAAGGGAAGC
481TGGTCATTGA TGAGCCAGCC AAGGAGAAGA ACGAGAAAGG AGCGTTGAAG AGGAGAGCAG
541GGGACTTGCT GGAGGACTCT CCTAAACGTC CCAAGGAGGC AGAAAACCCT GAAGGAGAGG
601AGAAGGAGGC AGCCACCTTG GAGGTTGAGA GGCCCCTTCC TATGGAGGTG GAAAAGAATA
661GCACCCCCTC TGAGCCCGGC TCTGGCCGGG GGCCTCCCCA AGAGGAAGAA GAAGAGGAGG
721ATGAAGAGGA AGAGGCTACC AAGGAAGATG CTGAGGCCCC AGGCATCAGA GATCATGAGA
781GCCTGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
841TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
901CTTGGCTTTA TATATCTTGT GGAAAGGACG AATTGACTAA TAATCTATTT ACCTCACGCG
961TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)