Construct: ORF TRCN0000470424
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004702.1_s317c1
- Derived from:
- ccsbBroadEn_06358
- DNA Barcode:
- ATTGACTAATAATCTATTTACCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HDGF (3068)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470424
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3068 | HDGF | heparin binding growth factor | NM_004494.3 | 99.7% | 100% | 270G>A;648G>A |
| 2 | human | 3068 | HDGF | heparin binding growth factor | NM_001126051.1 | 92.1% | 87.7% | (many diffs) |
| 3 | human | 3068 | HDGF | heparin binding growth factor | NM_001126050.2 | 88.2% | 81.5% | (many diffs) |
| 4 | human | 3068 | HDGF | heparin binding growth factor | NM_001319187.1 | 86.5% | 86.6% | 0_1ins96;174G>A |
| 5 | human | 3068 | HDGF | heparin binding growth factor | NM_001319188.1 | 86.5% | 86.6% | 0_1ins96;174G>A |
| 6 | human | 3068 | HDGF | heparin binding growth factor | XM_011509453.2 | 86.3% | 86.6% | 0_1ins96;174G>A;552G>A |
| 7 | human | 3068 | HDGF | heparin binding growth factor | XM_011509454.2 | 86.3% | 86.6% | 0_1ins96;174G>A;552G>A |
| 8 | human | 3068 | HDGF | heparin binding growth factor | XM_011509455.2 | 86.3% | 86.6% | 0_1ins96;174G>A;552G>A |
| 9 | human | 3068 | HDGF | heparin binding growth factor | NM_001319186.2 | 85.8% | 79.4% | (many diffs) |
| 10 | human | 3068 | HDGF | heparin binding growth factor | XR_001737133.1 | 31.9% | (many diffs) | |
| 11 | human | 3068 | HDGF | heparin binding growth factor | NR_135008.2 | 30.4% | (many diffs) | |
| 12 | mouse | 15191 | Hdgf | hepatoma-derived growth factor | NM_008231.4 | 88% | 88.3% | (many diffs) |
| 13 | mouse | 15191 | Hdgf | hepatoma-derived growth factor | NM_001331052.1 | 75.9% | 75% | (many diffs) |
| 14 | mouse | 15191 | Hdgf | hepatoma-derived growth factor | XM_006501029.3 | 75.9% | 75% | (many diffs) |
| 15 | mouse | 15191 | Hdgf | hepatoma-derived growth factor | NM_001331051.1 | 61.8% | 46.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 786
- ORF length:
- 720
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gcgatccaac cggcagaagg agtacaaatg cggggacctg gtgttcgcca 121 agatgaaggg ctacccacac tggccggccc ggattgacga gatgcctgag gctgccgtga 181 aatcaacagc caacaaatac caagtctttt ttttcgggac ccacgagacg gcattcctgg 241 gccccaaaga cctcttccct tacgaggaat ccaaggagaa gtttggcaag cccaacaaga 301 ggaaagggtt cagcgagggg ctgtgggaga tcgaaaaCAA CCCTACTGTC AAGGCTTCCG 361 GCTATCAGTC CTCCCAGAAA AAGAGCTGTG TGGAAGAGCC TGAACCAGAG CCCGAAGCTG 421 CAGAGGGTGA CGGTGATAAG AAGGGGAATG CAGAGGGCAG CAGCGACGAG GAAGGGAAGC 481 TGGTCATTGA TGAGCCAGCC AAGGAGAAGA ACGAGAAAGG AGCGTTGAAG AGGAGAGCAG 541 GGGACTTGCT GGAGGACTCT CCTAAACGTC CCAAGGAGGC AGAAAACCCT GAAGGAGAGG 601 AGAAGGAGGC AGCCACCTTG GAGGTTGAGA GGCCCCTTCC TATGGAGGTG GAAAAGAATA 661 GCACCCCCTC TGAGCCCGGC TCTGGCCGGG GGCCTCCCCA AGAGGAAGAA GAAGAGGAGG 721 ATGAAGAGGA AGAGGCTACC AAGGAAGATG CTGAGGCCCC AGGCATCAGA GATCATGAGA 781 GCCTGTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 841 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 901 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATTGACTAA TAATCTATTT ACCTCACGCG 961 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt