Construct: ORF TRCN0000470433

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003500.1_s317c1
Derived from:
ccsbBroadEn_15895
DNA Barcode:
CATTGAGCAGTGACATAAGTATGG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RHOT1 (55288)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470433
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55288 RHOT1 ras homolog family member T1 NM_018307.4 100% 100%
2 human 55288 RHOT1 ras homolog family member T1 NM_001288755.1 96.6% 96.6% 0_1ins63
3 human 55288 RHOT1 ras homolog family member T1 XM_017024825.1 96.6% 96.6% 0_1ins63
4 human 55288 RHOT1 ras homolog family member T1 XM_011524976.2 95.1% 94.3% (many diffs)
5 human 55288 RHOT1 ras homolog family member T1 NM_001288754.1 95% 95% 1740_1835del
6 human 55288 RHOT1 ras homolog family member T1 XM_017024823.1 94.2% 92.4% (many diffs)
7 human 55288 RHOT1 ras homolog family member T1 NM_001033566.2 93.7% 93.7% 1740_1862del
8 human 55288 RHOT1 ras homolog family member T1 XM_017024821.1 92.4% 90.2% (many diffs)
9 human 55288 RHOT1 ras homolog family member T1 XM_017024819.1 92.1% 91.9% (many diffs)
10 human 55288 RHOT1 ras homolog family member T1 XM_017024824.1 91.8% 91.8% 0_1ins63;1677_1772del
11 human 55288 RHOT1 ras homolog family member T1 XM_011524973.2 91.7% 89.3% (many diffs)
12 human 55288 RHOT1 ras homolog family member T1 XM_011524971.2 90.8% 88.2% (many diffs)
13 human 55288 RHOT1 ras homolog family member T1 XM_017024820.1 90.7% 89.7% (many diffs)
14 human 55288 RHOT1 ras homolog family member T1 XM_017024822.1 90.5% 90.5% 0_1ins63;1677_1799del
15 human 55288 RHOT1 ras homolog family member T1 NM_001033568.2 89.4% 89.4% 1740_1958del
16 human 55288 RHOT1 ras homolog family member T1 XM_011524975.3 86.3% 86.3% 0_1ins63;1677_1895del
17 human 55288 RHOT1 ras homolog family member T1 XM_024450827.1 86.3% 86.3% 0_1ins63;1677_1895del
18 human 55288 RHOT1 ras homolog family member T1 XM_011524969.2 85.6% 83.2% (many diffs)
19 human 55288 RHOT1 ras homolog family member T1 NM_001033567.2 79.4% 79.4% 0_1ins381
20 human 55288 RHOT1 ras homolog family member T1 NM_001288758.1 75.5% 75.5% 0_1ins381;1359_1454del
21 human 55288 RHOT1 ras homolog family member T1 XR_934499.2 63.1% 1_35del;1572_1623del;1942_2938del
22 human 55288 RHOT1 ras homolog family member T1 XR_001752556.1 60.5% (many diffs)
23 human 55288 RHOT1 ras homolog family member T1 XR_001752555.1 59.3% 1_35del;1775_2013del;2129_3125del
24 human 55288 RHOT1 ras homolog family member T1 XR_001752554.1 57% 0_1ins37;1703_2037del;2153_3149del
25 human 55288 RHOT1 ras homolog family member T1 NR_110083.1 54.5% 1_239del;1979_2239del;2355_3401del
26 mouse 59040 Rhot1 ras homolog family member T1 NM_021536.7 90.3% 95.8% (many diffs)
27 mouse 59040 Rhot1 ras homolog family member T1 NM_001163354.1 86% 91.2% (many diffs)
28 mouse 59040 Rhot1 ras homolog family member T1 NM_001163355.1 84.8% 90% (many diffs)
29 mouse 59040 Rhot1 ras homolog family member T1 XM_011249168.2 81% 85.9% (many diffs)
30 mouse 59040 Rhot1 ras homolog family member T1 XM_011249167.2 79.2% 84% (many diffs)
31 mouse 59040 Rhot1 ras homolog family member T1 XM_006533886.3 75.8% 80.4% (many diffs)
32 mouse 59040 Rhot1 ras homolog family member T1 XR_879726.2 69.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1923
ORF length:
1854
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaagaaagac gtgcggatcc tgctggtggg agaacctaga gttgggaaga
121catcactgat tatgtctctg gtcagtgaag aatttccaga agaggttcct ccccgggcag
181aagaaatcac cattccagct gatgtcaccc cagagagagt tccaacacac attgtagatt
241actcagaagc agaacagagt gatgaacaac ttcatcaaga aatatctcag gctaatgtca
301tctgtatagt gtatgccgtt aacaacaagc attctattga taaggtaaca agtcgatgga
361ttcctctcat aaatgaaaga acagacaaag acagcaggct gcctttaata ttggttggga
421acaaatctga tctggtggaa tatagtagta tggagaccat ccttcctatt atgaaccagt
481atacagaaat agaaacctgt gtggagtgtt cagcgaaaaa cctgaagaac atatcagagc
541tcttttatta cgcacagaaa gctgttcttc atcctacagg gcccctgtac tgcccagagg
601agaaggagat gaaaccagct tgtataaaag cccttactcg tatatttaaa atatctgatc
661aagataatga tggtactctc aatgatgctg aactcaactt ctttcagagg atttgtttca
721acactccatt agctcctcaa gctctggagg atgtcaagaa tgtagtcaga aaacatataa
781gtgatggtgt ggctgacagt gggttgaccc tgaaaggttt tctcttttta cacacacttt
841ttatccagag agggagacac gaaactactt ggactgtgct tcgacgattt ggttatgatg
901atgacctgga tttgacacct gaatatttgt tccccctgct gaaaatacct cctgattgca
961ctactgaatt aaatcatcat gcatatttat ttctccaaag cacctttgac aagcatgatt
1021tggatagaga ctgtgctttg tcacctgatg agcttaaaga tttatttaaa gttttccctt
1081acataccttg ggggccagat gtgaataaca cagtttgtac caatgaaaga ggctggataa
1141cctaccaggg attcctttcc cagtggacgc tcacgactta tttagatgta cagcggtgcc
1201tggaatattt gggctatcta ggctattcaa tattgactga gcaagagtct caagcttcag
1261ctgttacagt gacaagagat aaaaagatag acctgcagaa aaaacaaact caaagaaatg
1321tgttcagatg taatgtaatt ggagtgaaaa actgtgggaa aagtggagtt cttcaggctc
1381ttcttggaag aaacttaatg aggcagaaga aaattcgtga agatcataaa tcctactatg
1441cgattaacac tgtttatgta tatggacaag agaaatactt gttgttgcat gatatctcag
1501aatcggaatt tctaactgaa gctgaaaTCA TTTGTGATGT TGTATGCCTG GTATATGATG
1561TCAGCAATCC CAAATCCTTT GAATACTGTG CCAGGATTTT TAAGCAACAC TTTATGGACA
1621GCAGAATACC TTGCTTAATC GTAGCTGCAA AGTCAGACCT GCATGAAGTT AAACAAGAAT
1681ACAGTATTTC ACCTACTGAT TTCTGCAGGA AACACAAAAT GCCTCCACCA CAAGCCTTCA
1741CTTGCAATAC TGCTGATGCC CCCAGTAAGG ATATCTTTGT TAAATTGACA ACAATGGCCA
1801TGTATCCGCA CGTGACACAA GCTGACCTCA AGAGCTCCAC GTTTTGGCTT CGAGCAAGTT
1861TTGGTGCTAC TGTTTTTGCA GTTTTGGGCT TTGCTATGTA CAAAGCATTA TTGAAACAGC
1921GATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1981TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT GAAAGTATTT CGATTTCTTG
2041GCTTTATATA TCTTGTGGAA AGGACGACAT TGAGCAGTGA CATAAGTATG GACGCGTTAA
2101GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.