Construct: ORF TRCN0000470439
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003522.1_s317c1
- Derived from:
- BRDN0000394333
- DNA Barcode:
- CCTGACGACTCTCAATGGACTAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MEX3D (399664)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470439
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 399664 | MEX3D | mex-3 RNA binding family me... | XM_017026811.1 | 84.7% | 84.4% | (many diffs) |
| 2 | human | 399664 | MEX3D | mex-3 RNA binding family me... | NM_001174118.2 | 73.1% | 72.9% | 1_534del;554_555delTGinsAC;1601C>T |
| 3 | human | 399664 | MEX3D | mex-3 RNA binding family me... | NM_203304.3 | 70.4% | 70.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1533
- ORF length:
- 1464
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgagtgc gtcccggacc ccagctccga gcacgtcgcc gagatcgtgg 121 gtcgccaggg ctgcaagatc aaggccctgc gggccaagac aaacacctac atcaagaccc 181 cagtgcgggg cgaggagccg gtcttcatcg tgaccggccg gaaggaggac gtggagatgg 241 ccaagcgtga gatcctgtcg gcggccgaac acttctccat catccgcgcc acgcgcagca 301 aggccggggg tctgcccggc gccgcccagg gcccgcccaa ccttcccgga cagaccacca 361 tccaggtgcg cgtgccctac cgggtggtgg ggctggtggt ggggcccaag ggcgccacca 421 tcaagcgcat ccagcagcgg acgcacacct acatcgtgac gcccgggcgc gacaaggagc 481 cggtgttcgc ggtcactggg atgcccgaga acgtggaccg cgcgcgcgag gagatcgagg 541 cgcacatcac gctgcgcact ggcgccttca ccgacgcggg ccccgacagc gacttccacg 601 ccaacggcac cgacgtctgc ctggacctgc tcggggcggc cgccagcctc tgggccaaga 661 cccccaacca gggacgacgg ccccccacgg ccacggccgg cctccgcggg gacacggccc 721 tgggcgcccc cagcgccccc gaggccttct acgcgggcag ccgcggcggc ccctccgtgc 781 cggacccagg ccccgccagc ccctacagcg gctccggcaa cgggggcttc gccttcggcg 841 cggagggtcc cggtgccccg gtggggacgg ccgcccccga cgactgcgac ttcggcttcg 901 acttcgactt cctggcgctg gacctgaccg tgcccgccgc ggccaccatc tgggcgcctt 961 ttgagcgcgc cgcccccttg cccgccttca gcggctgctc cacggtcaac ggagccccgg 1021 gacctcccgc cgccggcgcc cggcgcagca gtggggccgg gaccccccgc cactcgccca 1081 cgctgcccga gcccggcggc ctccgcctgg agctcccgct gtctcgccgt ggcgtcccgg 1141 acccggtggg cgcgctgtcc tggcgacccc cgcagggccc cgtatccttc ccaggcggcg 1201 ccgccTTCTC CACGGCCACC TCGCTGCCCA GCAGCCCCGC GGCCGCCGCC TGCGCCCCCC 1261 TGGACTCCGG CGCCTCCGAG AACAGCCGCA AGCCCCCTTC GGCGTCCTCG GCCCCGGCCC 1321 TGGCGCGAGA GTGCGTGGTG TGCGCCGAGG GCGAGGTGAT GGCTGCGCTG GTCCCCTGCG 1381 GCCACAACCT CTTCTGCATG GACTGCGCCG TCCGCATCTG CGGCAAGAGC GAGCCCGAGT 1441 GTCCCGCCTG CCGCACGCCG GCCACCCAGG CCATTCGCGT GGAGACGGAG ACCCCGCAGC 1501 CCGGCGGCGC CTCAGCCCTT CAACGACAGT ATTTGCCAAC TTTCTTGTAC AAAGTGGTTG 1561 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1621 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 1681 TGACGACTCT CAATGGACTA GCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1741 ttgtgaaaga tt