Construct: ORF TRCN0000470454
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004858.1_s317c1
- Derived from:
- ccsbBroadEn_02385
- DNA Barcode:
- GACTTCTTGCCTGACTATCGTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BCAS2 (10286)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470454
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10286 | BCAS2 | BCAS2 pre-mRNA processing f... | NM_005872.3 | 100% | 100% | |
| 2 | mouse | 68183 | Bcas2 | breast carcinoma amplified ... | NM_026602.3 | 89% | 100% | (many diffs) |
| 3 | mouse | 68183 | Bcas2 | breast carcinoma amplified ... | XM_006501965.1 | 82% | 92.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 741
- ORF length:
- 675
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggcacaggt ttggtggctg gagaggttgt ggtggatgcg ctgccgtatt 121 ttgatcaagg ttatgaagcc cctggtgtgc gggaagcggc tgcagcgctg gtggaggagg 181 aaactcgcag ataccgacct actaagaact acctgagcta cctgacagcc ccggattatt 241 ctgcctttga aactgacata atgagaaatg aatttgaaag actGGCTGCT CGACAACCAA 301 TTGAATTGCT CAGTATGAAA CGATATGAGC TTCCAGCCCC CTCCTCTGGT CAAAAAAATG 361 ACATTACTGC ATGGCAAGAA TGTGTAAACA ATTCTATGGC CCAGTTAGAG CATCAAGCAG 421 TTAGAATTGA GAATCTGGAA CTAATGTCAC AGCATGGATG TAATGCCTGG AAAGTATACA 481 ATGAAAATCT AGTTCATATG ATTGAACACG CACAGAAGGA ACTTCAGAAG TTAAGAAAAC 541 ATATTCAAGA TTTAAACTGG CAGAGAAAGA ACATGCAACT CACAGCTGGA TCTAAATTGA 601 GAGAAATGGA GTCAAATTGG GTATCCCTGG TCAGTAAGAA TTATGAGATT GAACGGACTA 661 TTGTTCAGCT AGAAAATGAA ATCTATCAAA TTAAGCAGCA ACATGGAGAG GCAAACAAAG 721 AAAACATCCG GCAAGACTTC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 781 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 841 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGACT TCTTGCCTGA 901 CTATCGTGCC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt