Construct: ORF TRCN0000470458
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005565.1_s317c1
- Derived from:
- ccsbBroadEn_02491
- DNA Barcode:
- CTAAAGTGTAAATATCATCTACGG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RAD51AP1 (10635)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470458
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10635 | RAD51AP1 | RAD51 associated protein 1 | NM_006479.5 | 99.9% | 67.4% | 670_671insC |
| 2 | human | 10635 | RAD51AP1 | RAD51 associated protein 1 | NM_001130862.2 | 95% | 64.2% | 210_260del;721_722insC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 765
- ORF length:
- 699
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gcggcctgtg agacataaga aaccagtcaa ttactcacag tttgaccact 121 ctgacagtga tgatgatttt gtttctgcaa ctgtaccttt aaacaagaaa tccagaacag 181 caccaaagga gttaaaacaa gataaaccaa aacctaactt gaacaatctc cggaaagaag 241 aaatcccagt acaagagaaa acccctaaaa aaaggatggc tttagatgac aagctctacc 301 agagagactt agaagttgca ctagctttat cagtgaagga acttccaaca gtcaccacta 361 atgtgcagaa ctctcaagat aaaagcattg aaaaacatgg cagtagtaaa atagaaacaa 421 tgaataagtc tcctcatatc tctaattgca gtgtagccag tgattattta gatttggata 481 agattactgt ggaagatgat gttggtggtg ttcaagggaa aagaaaagca gcatctaaag 541 ctgcagcaca gcagaggaag attcttctgg aaggcagtga tggtgatagt gctaatgaca 601 ctgaaccaga ctttgcacct ggtgaagatt ctgaggatga ttctgatttt tgtgagagtg 661 aggataatga cgaagacttc tctatgagaa aaagtaaagt taaagaaatt aaaaagaaag 721 aagtgaaggT AAAATCCCCC AGTAGAAAAG AAAGAGAAGA AATCTAAATC CAAATGTAAT 781 GCTTTGGTGA CTTCGGTGGA CTCTGCTCCA GCTGCCGTCA AATCAGAATC TCAGTCCTTG 841 CCAAAAAAGG TTTCTCTGTC TTCAGATACC ACTAGGAAAC CATTAGAAAT ACGCAGTCCT 901 TCAGCTGAAA GCAAGAAACC TAAATGGGTC CCACCAGCGG CATCTGGAGG TAGCAGAAGT 961 AGCAGCAGCC CACTGGTGGT AGTGTCTGTG AAGTCTCCCA ATCAGAGTCT CCGCCTTGGC 1021 TTGTCCAGAT TAGCACGAGT TAAACCTTTG CATCCAAATG CCACTAGCAC CTGCCCAACT 1081 TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT 1141 TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA 1201 TCTTGTGGAA AGGACGACTA AAGTGTAAAT ATCATCTACG GACGCGTTAA GTCgacaatc 1261 aacctctgga ttacaaaatt tgtgaaagat t