Construct: ORF TRCN0000470465
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009272.1_s317c1
- Derived from:
- ccsbBroadEn_15812
- DNA Barcode:
- AGCGCATCGCTCAATGGACGGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF8 (50717)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470465
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50717 | DCAF8 | DDB1 and CUL4 associated fa... | NR_028106.1 | 54.7% | 1_279del;483C>G;1099_1495del | |
| 2 | human | 50717 | DCAF8 | DDB1 and CUL4 associated fa... | NR_028105.1 | 47.3% | 1_512del;716C>G;1332_1728del | |
| 3 | human | 50717 | DCAF8 | DDB1 and CUL4 associated fa... | NM_015726.4 | 44.3% | 41.6% | (many diffs) |
| 4 | human | 50717 | DCAF8 | DDB1 and CUL4 associated fa... | NR_028104.2 | 19.2% | (many diffs) | |
| 5 | human | 50717 | DCAF8 | DDB1 and CUL4 associated fa... | NR_028103.2 | 18.9% | (many diffs) | |
| 6 | mouse | 98193 | Dcaf8 | DDB1 and CUL4 associated fa... | NM_153555.2 | 39.1% | 37.9% | (many diffs) |
| 7 | mouse | 98193 | Dcaf8 | DDB1 and CUL4 associated fa... | XM_006497065.3 | 39.1% | 37.9% | (many diffs) |
| 8 | mouse | 98193 | Dcaf8 | DDB1 and CUL4 associated fa... | XM_011238889.2 | 39.1% | 37.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 885
- ORF length:
- 819
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc cagcaaaggg agcagcacag atggcagaac agacttagct aatggaagcc 121 tgtctagcag tccagaggag atgtctggag ctgaagaggg gagggagaca tcctcaggca 181 ttgaagtgga ggcctcagac ctgagtttga gcttgactgg ggatgatggt ggccccaacc 241 gcaccagcac agaaagtcga ggcacagaga cagagagctc aggtgaagat aaggactctg 301 acagcatgga ggacactggt cattactcca ttaatgatga aaatcgagtc catgaccgct 361 cagaggaaga ggaagaggag gaagaagagg aggaagaaga gcagcctcgg cgccgtgtac 421 agcgcaagcg ggctaaccgt gaccaggact catcagatga tgagcgggcc ctagaggact 481 gggtgtcctc agaaacatca gctctacccc gacctcgctg gcaagccctc cctgcccTTC 541 GGGAGCGGGA GCTGGGTTCA AGTGCCCGCT TTGTCTATGA GGCCTGTGGG GCAAGAGTCT 601 TTGTGCAGCG TTTCCGCCTG CAGCATGGGC TTGAGGGCCA TACTGGTTGT GTCAATACCC 661 TGCACTTTAA CCAGCGCGGC ACCTGGCTGG CCAGTGGCAG CGATGACCTG AAGGTGGTGG 721 TGTGGGATTG GGTACGGCGG CAGCCAGTAC TGGACTTTGA GAGTGGCCAC AAAAGTAATG 781 TGTTCCAGGT GAGGCAAGGG AGCATAATAG CAACTGAGAG AATCAGGCAT GAGTTAGAGA 841 AATCTGAACT GCAGCATGGA TTGGGAGCTG ATAGTGAGTT ATTGTACCCA ACTTTCTTGT 901 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 961 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1021 GAAAGGACGA AGCGCATCGC TCAATGGACG GCCGACGCGT TAAGTCgaca atcaacctct 1081 ggattacaaa atttgtgaaa gatt