Construct: ORF TRCN0000470473
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000839.1_s317c1
- Derived from:
- ccsbBroadEn_08717
- DNA Barcode:
- TTCATAAACATATGGGTGTCTATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPRYD7 (57213)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470473
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57213 | SPRYD7 | SPRY domain containing 7 | NM_001127482.2 | 99.7% | 99.3% | 377T>A |
| 2 | human | 57213 | SPRYD7 | SPRY domain containing 7 | NM_020456.4 | 79.9% | 79.5% | 106_222del;494T>A |
| 3 | human | 57213 | SPRYD7 | SPRY domain containing 7 | XM_017020677.1 | 67.7% | 64.7% | (many diffs) |
| 4 | human | 57213 | SPRYD7 | SPRY domain containing 7 | XR_001749634.1 | 14% | (many diffs) | |
| 5 | human | 57213 | SPRYD7 | SPRY domain containing 7 | NR_023351.3 | 10.3% | (many diffs) | |
| 6 | mouse | 66674 | Spryd7 | SPRY domain containing 7 | XM_011245148.1 | 75.7% | 79.2% | (many diffs) |
| 7 | mouse | 66674 | Spryd7 | SPRY domain containing 7 | NM_025697.4 | 72.9% | 78% | (many diffs) |
| 8 | mouse | 66674 | Spryd7 | SPRY domain containing 7 | NM_001310603.1 | 56.5% | 44% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 537
- ORF length:
- 471
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cacctcggtg ttgtgctgcc tgcggtgctg cagagacggg gggactggcc 121 acatcccTCT GAAGGAGATG CCGGCCGTGC AGCTGGACAC GCAGCACATG GGAATCTGGG 181 GTATTGGTGT TGCAACTCAG AAGGTTAACT TGAATCAGAT TCCTCTTGGC CGAGATATGC 241 ACAGTCTGGT GATGAGAAAT GATGGAGCCC TTTACCACAA CAATGAAGAG AAAAATAGGC 301 TGCCAGCAAA CAGTCTTCCG CAGGAAGGAG ATGTGGTGGG TATTACTTAT GACCATGTCG 361 AATTAAATGT ATACTTGAAT GGAAAAAACA TGCATTGTCC AGCATCAGGT ATACGAGGGA 421 CAGTGTATCC AGTTGTTTAT GATGATGACA GTGCAATTTT GGATTGCCAG TTCAGTGAGT 481 TTTATCATAC GCCTCCACCT GGTTTTGAAA AAATATTATT TGAACAGCAA ATCTTCTGCC 541 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 601 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 661 ATATATCTTG TGGAAAGGAC GATTCATAAA CATATGGGTG TCTATCACGC GTTAAGTCga 721 caatcaacct ctggattaca aaatttgtga aagatt