Construct: ORF TRCN0000470474
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013079.1_s317c1
- Derived from:
- ccsbBroadEn_10224
- DNA Barcode:
- GTTGTTAATTAATCCACACCCTTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FTH1P3 (2498)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470474
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2495 | FTH1 | ferritin heavy chain 1 | NM_002032.3 | 32.8% | 31.4% | (many diffs) |
| 2 | human | 2498 | FTH1P3 | ferritin heavy chain 1 pseu... | NR_002201.1 | 32.3% | 1_119del;429_954del | |
| 3 | mouse | 14319 | Fth1 | ferritin heavy polypeptide 1 | NM_010239.2 | 30% | 30.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 91
- ORF end:
- 400
- ORF length:
- 309
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTTCTTC CATTTCAGGT GTCGTGAGGC TAGCATCGAT 61 TGATCAACAA GTTTGTACAA AAAAGTTGGC ATGCCCTCGG ACTGCCCCAA GGCCCCCGCC 121 GCAGCTCCAG CGCCGCGTAG CCACCACTGC CGCTGCCGCC GCCTCTCCTT AGTCGCCGGC 181 ATGACGACCG CGTCTACCTC GCAGGTGCGC CAGAACTACC ACCAAGACTC AGAGGCCGCC 241 ATCAACCGCC AGATCAACCT GGAGCTCTAC GCCTCCTCCA TTTACCTGTG CGTGGCTTAC 301 TACTTTGACA GCGATGATGT GGCTTTGAAG AACTTTGCCA AATACTTTCT TCACCAATCT 361 CATGAGGAGA GGGAACATGC TGAGAAATTG ATGAAGCTGT ACCCAACTTT CTTGTACAAA 421 GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA 481 TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG 541 GACGAGTTGT TAATTAATCC ACACCCTTGA CGCGTTAAGT Cgacaatcaa cctctggatt 601 acaaaatttg tgaaagatt